PMID- 17120813
OWN - NLM
STAT- MEDLINE
DCOM- 20070830
LR  - 20191110
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 29
IP  - 15
DP  - 2006 Oct
TI  - High performance liquid chromatography for the determination of glucosamine 
      sulfate in human plasma after derivatization with 9-fluorenylmethyl 
      chloroformate.
PG  - 2296-302
AB  - In this study, we developed a simple, rapid, sensitive, and reliable method for 
      the determination of glucosamine sulfate in human plasma, which was based on 
      derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by 
      reverse-phase HPLC-FLD. For the first time, FMOC-Cl was introduced into 
      derivatization of glucosamine sulfate in human plasma. The amino groups of 
      glucosamine sulfate and vertilmicin sulfate (the internal standard) were trapped 
      with FMOC-Cl to form glucosamine-FMOC-Cl and vertilmicin-FMOC-Cl adducts, which 
      can be very suitable for HPLC-FLD. Precipitation of plasma proteins by 
      acetonitrile was followed by vortex mixing and centrifugation. Chromatographic 
      separation was performed on a C18 column (DIAMONSIL 150 x 4 mm id, 5 microm) with 
      a mobile phase gradient consisting of acetonitrile and water at a flow-rate of 1 
      mL/min. The retention times of glucosamine-FMOC-Cl and vertilmicin-FMOC-Cl 
      adducts were 8.9 and 21.2 min, respectively. This method was shown to be 
      selective and sensitive for glucosamine sulfate. The limit of detection was 15 
      ng/mL for glucosamine sulfate in plasma and the linear range was 0.1-10 mg/mL in 
      plasma with a correlation coefficient (r) of 0.9999. The relative standard 
      deviations (RSDs) of intra-day and inter-day assays were 5.2-8.1% and 6.1- 8.5%, 
      respectively. Extraction recoveries of glucosamine sulfate in plasma were greater 
      than 90%. The validated method was successfully applied to the determination of 
      glucosamine sulfate in human plasma samples.
FAU - Huang, Tao-min
AU  - Huang TM
AD  - Department of Pharmacy, Fudan University, Shanghai, PR China.
FAU - Deng, Chun-Hui
AU  - Deng CH
FAU - Chen, Nian-zhu
AU  - Chen NZ
FAU - Liu, Zhen
AU  - Liu Z
FAU - Duan, Geng-Li
AU  - Duan GL
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Fluorenes)
RN  - 0 (Indicators and Reagents)
RN  - 9PLB0BTT90 (1-(9-fluorenyl)methyl chloroformate)
RN  - N08U5BOQ1K (Glucosamine)
SB  - IM
MH  - Blood Chemical Analysis/methods/statistics & numerical data
MH  - Chromatography, High Pressure Liquid/*methods/statistics & numerical data
MH  - Dietary Supplements/analysis
MH  - Fluorenes
MH  - Glucosamine/administration & dosage/*blood
MH  - Humans
MH  - Indicators and Reagents
MH  - Sensitivity and Specificity
EDAT- 2006/11/24 09:00
MHDA- 2007/08/31 09:00
CRDT- 2006/11/24 09:00
PHST- 2006/11/24 09:00 [pubmed]
PHST- 2007/08/31 09:00 [medline]
PHST- 2006/11/24 09:00 [entrez]
AID - 10.1002/jssc.200600162 [doi]
PST - ppublish
SO  - J Sep Sci. 2006 Oct;29(15):2296-302. doi: 10.1002/jssc.200600162.