PMID- 17122104
OWN - NLM
STAT- MEDLINE
DCOM- 20070201
LR  - 20131121
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 47
IP  - 12
DP  - 2006 Dec
TI  - Prolonged rhodopsin phosphorylation in light-induced retinal degeneration in rat 
      models.
PG  - 5204-11
AB  - PURPOSE: The effects of various light-induced stresses on the retina were 
      examined in the retinal degenerative rat model. METHODS: Retinal morphology and 
      electroretinograms (ERGs) were analyzed after application of light-induced stress 
      of several intensities (650, 1300, 2500, or 5000 lux). For evaluation of 
      rhodopsin (Rho) function, the kinetics of Rho regeneration and dephosphorylation 
      were studied by spectrophotometric analysis and immunofluorescence labeling with 
      antibodies specifically directed toward the phosphorylated residues (334)Ser and 
      (338)Ser in the C terminus of Rho. Retinal cGMP concentration was determined by 
      ELISA. Expression levels of neurotrophic factors (FGF2, brain-derived 
      neurotrophic factor [BDNF], platelet-derived growth factor [PDGF], and ciliary 
      neurotrophic factor [CNTF]) were evaluated quantitatively by RT-PCR. RESULTS: 
      Light intensity-dependent deterioration of ERG responses and thinning of the 
      retinal outer nuclear layer were observed in wild-type and Royal College of 
      Surgeons (RCS) rat retinas. Under dark adaptation after light-induced stress, the 
      kinetics of Rho regeneration were not different between wild-type and RCS rat 
      retinas. Rho dephosphorylation at (334)Ser and (338)Ser was extremely delayed in 
      RCS rat retinas compared with wild-type without light-induced stress, but Rho 
      dephosphorylation at those sites became slower in both RCS and wild-type rat 
      retinas. In terms of expression of neurotrophic factors, almost no significant 
      changes were observed between the animals after light-induced stress. 
      CONCLUSIONS: The present study indicates that light-induced stress causes 
      intensity-dependent deterioration in retinal function and morphology in wild-type 
      and RCS rat retinas. Disruption of the phototransduction cascade resulting from 
      slower kinetics of Rho dephosphorylation appears to be involved in retinal 
      degeneration.
FAU - Ishikawa, Futoshi
AU  - Ishikawa F
AD  - Department of Ophthalmology, Hirosaki University School of Medicine, Hirosaki, 
      Japan.
FAU - Ohguro, Hiroshi
AU  - Ohguro H
FAU - Ohguro, Ikuyo
AU  - Ohguro I
FAU - Yamazaki, Hitoshi
AU  - Yamazaki H
FAU - Mamiya, Kazuhisa
AU  - Mamiya K
FAU - Metoki, Tomomi
AU  - Metoki T
FAU - Ito, Tadashi
AU  - Ito T
FAU - Yokoi, Yumiko
AU  - Yokoi Y
FAU - Nakazawa, Mitsuru
AU  - Nakazawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 9009-81-8 (Rhodopsin)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Animals
MH  - Cyclic GMP/metabolism
MH  - Dark Adaptation
MH  - Electroretinography/radiation effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Light
MH  - Microscopy, Fluorescence
MH  - Nerve Growth Factors/genetics
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Radiation Injuries, Experimental/etiology/*metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Mutant Strains
MH  - Rats, Sprague-Dawley
MH  - Regeneration
MH  - Retina/metabolism/*radiation effects
MH  - Retinal Degeneration/etiology/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhodopsin/*metabolism
EDAT- 2006/11/24 09:00
MHDA- 2007/02/03 09:00
CRDT- 2006/11/24 09:00
PHST- 2006/11/24 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/11/24 09:00 [entrez]
AID - 47/12/5204 [pii]
AID - 10.1167/iovs.05-1149 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5204-11. doi: 10.1167/iovs.05-1149.