PMID- 17122633
OWN - NLM
STAT- MEDLINE
DCOM- 20070123
LR  - 20191210
IS  - 1541-2016 (Print)
IS  - 1533-4058 (Linking)
VI  - 14
IP  - 4
DP  - 2006 Dec
TI  - Refinement of immunohistologic parameters for Her2/neu scoring validation by FISH 
      and CISH.
PG  - 384-9
AB  - The conventional method of scoring Her2/neu immunostaining is recognized to 
      result in a high false-positive rate among 2+ cases when compared with results 
      obtained with fluorescence in situ hybridization (FISH); however, costs and 
      convenience dictates that immunohistochemistry remains the screening test for 
      Her2/neu status in patients with breast cancer. We describe refined criteria for 
      scoring of Her2/neu on the basis of anatomic localization rather than the 
      subjective assessment of intensity. The presence of a circumferential tram track 
      pattern that results from the staining of apposing cell membranes in >25% of the 
      tumor cells was necessary for a 3+ score (Her2/neu overexpressed) and the 
      presence of the tram track pattern in <25% was scored 2+ (equivocal); granular 
      and fragmented membrane staining was scored 1+ (negative). The tram track pattern 
      of Her2/neu overexpression showed 100% concordance with gene amplification. FISH 
      and CISH testing in selected cases from the other categories validated the 
      revised scoring method. These criteria reduced the numbers of equivocal staining 
      cases that required FISH testing.
FAU - Leong, Anthony S-Y
AU  - Leong AS
AD  - Immunohistology Unit, Division of Anatomical Pathology, Hunter Area Pathology 
      Service, Newcastle, Australia. aleong@mail.Newcastle.edu.au
FAU - Formby, Mark
AU  - Formby M
FAU - Haffajee, Zenobia
AU  - Haffajee Z
FAU - Clarke, Megan
AU  - Clarke M
FAU - Morey, Adrienne
AU  - Morey A
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/*metabolism
MH  - Breast Neoplasms/metabolism/*pathology
MH  - Cell Membrane/metabolism
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/*metabolism
EDAT- 2006/11/24 09:00
MHDA- 2007/01/24 09:00
CRDT- 2006/11/24 09:00
PHST- 2006/11/24 09:00 [pubmed]
PHST- 2007/01/24 09:00 [medline]
PHST- 2006/11/24 09:00 [entrez]
AID - 00129039-200612000-00003 [pii]
AID - 10.1097/01.pai.0000210415.53493.d4 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2006 Dec;14(4):384-9. doi: 
      10.1097/01.pai.0000210415.53493.d4.