PMID- 17123754
OWN - NLM
STAT- MEDLINE
DCOM- 20070221
LR  - 20131121
IS  - 0378-4274 (Print)
IS  - 0378-4274 (Linking)
VI  - 168
IP  - 1
DP  - 2007 Jan 10
TI  - Differential effects of cadmium on the gene expression of 
      seven-transmembrane-spanning receptors and GAPDH in the rat testis.
PG  - 51-7
AB  - Cadmium (Cd) is a widely spread toxicant with endocrine disrupting properties. 
      Under experimental conditions it suppresses sex steroid synthesis in the male as 
      well as the female. Testicular steroidogenesis is primarily regulated by 
      gonadotropins, but is also influenced by catecholamines. We have previously shown 
      that Cd exposure affects rat testosterone synthesis by down-regulating 
      luteinizing hormone (LH) receptor mRNA expression. In this study, rats were given 
      10 micromol/kg Cd subcutaneously and sacrificed 0.48-144 h later. We investigated 
      the effects of Cd on testicular gene expression of two adrenergic receptors. In 
      addition, mRNA levels of the androgen-regulated house keeping gene 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were measured. In contrast to 
      the suppressive influence on LH receptor expression Cd lacked effect on the 
      expression of alpha(1A)- and beta(2)-adrenergic receptors. GAPDH gene expression, 
      on the other hand, was up-regulated 1.6-fold after exposure to 10 micromol/kg Cd. 
      These data suggest that the influence of Cd on testicular gene expression 
      involves a specific effect on the LH receptor and not a general effect on 
      seven-transmembrane-spanning receptors. Also, data indicate that the increased 
      expression of GAPDH may be secondary to Cd-induced testosterone deprivation, 
      suggesting future studies of androgen-regulated genes in the toxicity of Cd.
FAU - Gunnarsson, David
AU  - Gunnarsson D
AD  - Department of Molecular Biology, Umea University, S-901 87 Umea, Sweden. 
      david.gunnarsson@molbiol.umu.se
FAU - Nordberg, Gunnar
AU  - Nordberg G
FAU - Selstam, Gunnar
AU  - Selstam G
LA  - eng
PT  - Journal Article
DEP - 20061110
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Adra1a protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Adrenergic, alpha-1)
RN  - 0 (Receptors, Adrenergic, beta-2)
RN  - 00BH33GNGH (Cadmium)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Cadmium/*toxicity
MH  - Gene Expression/*drug effects
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*genetics
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Adrenergic, alpha-1/genetics
MH  - Receptors, Adrenergic, beta-2/genetics
MH  - Testis/*drug effects/metabolism
EDAT- 2006/11/25 09:00
MHDA- 2007/02/22 09:00
CRDT- 2006/11/25 09:00
PHST- 2006/05/17 00:00 [received]
PHST- 2006/10/26 00:00 [revised]
PHST- 2006/10/27 00:00 [accepted]
PHST- 2006/11/25 09:00 [pubmed]
PHST- 2007/02/22 09:00 [medline]
PHST- 2006/11/25 09:00 [entrez]
AID - S0378-4274(06)01315-4 [pii]
AID - 10.1016/j.toxlet.2006.10.015 [doi]
PST - ppublish
SO  - Toxicol Lett. 2007 Jan 10;168(1):51-7. doi: 10.1016/j.toxlet.2006.10.015. Epub 
      2006 Nov 10.