PMID- 17123941
OWN - NLM
STAT- MEDLINE
DCOM- 20070820
LR  - 20171116
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 76
IP  - 3
DP  - 2007 Mar
TI  - Receptor-determined susceptibility of preimplantation embryos to pseudorabies 
      virus and porcine reproductive and respiratory syndrome virus.
PG  - 415-23
AB  - In the present study, the in vitro interaction of embryos with pseudorabies virus 
      (PRV) and porcine reproductive and respiratory syndrome virus (PRRSV) was 
      investigated by viral antigen detection and by evaluating the expression of virus 
      receptors, namely, poliovirus receptor-related 1 (PVRL1; formerly known as nectin 
      1) for PRV and sialoadhesin for PRRSV. Embryonic cells of zona pellucida intact 
      embryos incubated with PRV remained negative for viral antigens. Also, no 
      antigen-positive cells could be detected after PRV incubation of protease-treated 
      embryos, since the protease disrupted the expression of PRVL1. However, starting 
      from the five-cell-stage onwards, viral antigen-positive cells were detected 
      after subzonal microinjection of PRV. At this stage, the first foci of PVRL1, 
      also a known cell adhesion molecule, were expressed. At the expanded blastocyst 
      stage, a lining pattern of PVRL1 in the apicolateral border of trophectoderm 
      cells was present, whereas the expression in the inner cell mass was low. 
      Furthermore, PVRL1-specific monoclonal antibody CK41 significantly blocked PRV 
      infection of trophectoderm cells of hatched blastocysts, while the infection of 
      the inner cell mass was only partly inhibited. Viral antigen-positive cells were 
      never detected after PRRSV exposure of preimplantation embryos up to the hatched 
      blastocyst stage. Also, expression of sialoadhesin in these embryonic stages was 
      not detected. We conclude that the use of protease to investigate the virus 
      embryo interaction can lead to misinterpretation of results. Results also show 
      that blastomeres of five-cell embryos up to the hatched blastocysts can become 
      infected with PRV, but there is no risk of a PRRSV infection.
FAU - Mateusen, B
AU  - Mateusen B
AD  - Department of Reproduction, Obstetrics, and Herd Health, Faculty of Veterinary 
      Medicine, Ghent University, 9820 Merelbeke, Belgium. bart.mateusen@ugent.be
FAU - Van Soom, A
AU  - Van Soom A
FAU - Maes, D G D
AU  - Maes DG
FAU - Favoreel, H
AU  - Favoreel H
FAU - Nauwynck, H J
AU  - Nauwynck HJ
LA  - eng
PT  - Journal Article
DEP - 20061122
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Nectins)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Virus)
RN  - 0 (Sialic Acid Binding Ig-like Lectin 1)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/pharmacology
MH  - Blastocyst/drug effects/*physiology/*virology
MH  - Blastomeres/virology
MH  - Cell Adhesion Molecules/metabolism
MH  - Disease Susceptibility
MH  - Female
MH  - Herpesvirus 1, Suid/*pathogenicity
MH  - Male
MH  - Membrane Glycoproteins/metabolism
MH  - Nectins
MH  - Porcine Reproductive and Respiratory Syndrome/embryology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*pathogenicity
MH  - Pregnancy
MH  - Pseudorabies/embryology/virology
MH  - Receptors, Immunologic/metabolism
MH  - Receptors, Virus/*metabolism
MH  - Sialic Acid Binding Ig-like Lectin 1
MH  - Swine
MH  - Swine Diseases/virology
EDAT- 2006/11/25 09:00
MHDA- 2007/08/21 09:00
CRDT- 2006/11/25 09:00
PHST- 2006/11/25 09:00 [pubmed]
PHST- 2007/08/21 09:00 [medline]
PHST- 2006/11/25 09:00 [entrez]
AID - biolreprod.106.056697 [pii]
AID - 10.1095/biolreprod.106.056697 [doi]
PST - ppublish
SO  - Biol Reprod. 2007 Mar;76(3):415-23. doi: 10.1095/biolreprod.106.056697. Epub 2006 
      Nov 22.