PMID- 17124584
OWN - NLM
STAT- MEDLINE
DCOM- 20070622
LR  - 20240426
IS  - 0340-7004 (Print)
IS  - 1432-0851 (Electronic)
IS  - 0340-7004 (Linking)
VI  - 56
IP  - 7
DP  - 2007 Jul
TI  - Comprehensive epitope mapping of the Epstein-Barr virus latent membrane protein-2 
      in normal, non tumor-bearing individuals.
PG  - 1047-63
AB  - Latent membrane protein (LMP)-2 is one of the Epstein-Barr virus (EBV)-encoded 
      proteins consistently expressed by nasopharyngeal carcinoma (NPC). 
      EBV-transformed lymphoblastoid cell lines (LCL) have been used in patients with 
      NPC to induce LMP-2-recognizing T cell lines which have been in turn utilized for 
      protein-wide mapping of T cell epitopes. However, comprehensive mapping of 
      naturally recognized LMP-2 epitopes in non tumor-bearing individuals has not been 
      reported. Here, we applied a low sensitivity epitope-defining technique for the 
      identification of LMP-2 CTL responses detectable ex vivo in EBV-experienced 
      individuals. This screening tool has been previously validated by analyzing 
      memory CTL responses to Flu, cytomegalovirus (CMV), and the melanoma associated 
      antigen gp100/Mel17. Peripheral blood monocytes (PBMC) from ten Caucasian and ten 
      Chinese individuals were stimulated ex vivo with pools of nonamer (9-mer) 
      peptides overlapping in a stepwise fashion each single amino acid of the LMP-2 
      sequence. No obvious differences were observed between the immune response of the 
      two ethnic groups save for those related to the divergence in the ethnic 
      prevalence of HLA haplotypes. Several novel and known LMP-2 epitopes were 
      identified. Reactivity toward at least one LMP-2 epitope was detected in 18 of 
      the 20 donors but no prevalent human leukocyte antigen (HLA)/epitope combination 
      was observed confirming that LMP-2 reactivity in the context of common HLA 
      alleles is more pleiotropic than that of FLU and CMV. We believe that the 
      usefulness of these epitopes occurring naturally in non-cancer bearing patients 
      as reagents for the immunization of patients with early or advanced stage NPC 
      deserves further evaluation.
FAU - Provenzano, Maurizio
AU  - Provenzano M
AD  - Immune Oncology Section, Department of Surgery, University Hospital ZLF, 
      Hebelstrasse 20, 4031 Basel, Switzerland.
FAU - Selleri, Silvia
AU  - Selleri S
FAU - Jin, Ping
AU  - Jin P
FAU - Wang, Ena
AU  - Wang E
FAU - Werden, Rosemary
AU  - Werden R
FAU - Slezak, Stephanie
AU  - Slezak S
FAU - Adams, Sharon D
AU  - Adams SD
FAU - Panelli, Monica C
AU  - Panelli MC
FAU - Leitman, Susan F
AU  - Leitman SF
FAU - Stroncek, David F
AU  - Stroncek DF
FAU - Marincola, Francesco M
AU  - Marincola FM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061124
PL  - Germany
TA  - Cancer Immunol Immunother
JT  - Cancer immunology, immunotherapy : CII
JID - 8605732
RN  - 0 (DNA Primers)
RN  - 0 (EBV-associated membrane antigen, Epstein-Barr virus)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Viral Matrix Proteins)
RN  - 77238-31-4 (Interferon-beta)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Asian People
MH  - Cluster Analysis
MH  - DNA Primers
MH  - *Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*genetics
MH  - HLA Antigens
MH  - Herpesvirus 4, Human
MH  - Humans
MH  - Interferon-beta/metabolism
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/physiology
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Viral Matrix Proteins/*genetics
PMC - PMC11031044
EDAT- 2006/11/25 09:00
MHDA- 2007/06/23 09:00
PMCR- 2006/11/24
CRDT- 2006/11/25 09:00
PHST- 2006/08/14 00:00 [received]
PHST- 2006/10/17 00:00 [accepted]
PHST- 2006/11/25 09:00 [pubmed]
PHST- 2007/06/23 09:00 [medline]
PHST- 2006/11/25 09:00 [entrez]
PHST- 2006/11/24 00:00 [pmc-release]
AID - 246 [pii]
AID - 10.1007/s00262-006-0246-3 [doi]
PST - ppublish
SO  - Cancer Immunol Immunother. 2007 Jul;56(7):1047-63. doi: 
      10.1007/s00262-006-0246-3. Epub 2006 Nov 24.