PMID- 17124690
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20161021
IS  - 1300-6320 (Print)
IS  - 1300-6320 (Linking)
VI  - 29
IP  - 4
DP  - 2005
TI  - [The importance of antinuclear (ABA) and anti-double stranded dna (anti-dsDNA) 
      antibodies in the diagnosis of connective tissue diseases.].
PG  - 287-90
AB  - Connective tissue diseases (CTD) are characterized by the presence of 
      autoantibodies against several tissues. These autoantibodies occur against cell 
      membrane, cell receptors, plasma proteins, and cytoplasmic and nuclear 
      components. In laboratories, anti-nuclear antibodies (ANA) and anti-double 
      stranded DNA (anti-dsDNA) antibodies are widely used in diagnosis of CTD. The aim 
      of this study was to investigate the presence and accompaniment of ANA and 
      anti-dsDNA antibodies in diagnosis of several CTD and also to study the 
      prevalence of ANA and anti-dsDNA in a group of 88 patients with various types of 
      CTD. ANA were detected by immunofluorescence (IFA) using HEp-2 cells (Zeus 
      Scientific, Inc. USA) and anti-dsDNA antibodies using Crithidia luciliae 
      (BioSystems, Spain) as substrates in immunofluorescence. ANA Western Blot (WB) 
      Immunoassay (ImmuBlot, International Immuno-Diagnostics, USA) was also used along 
      with the tests referred to previously. ANA was found in the sera of 84 (96.5%) 
      patients while anti-dsDNA was detected in 7 (7.95%). Moreover different 
      fluorescence patterns were also evaluated with ANA IFA in accordance with 
      anti-dsDNA results. Mixed patterns in three and a homogeneous pattern in four 
      anti-dsDNA positive patients' sera were determined on HEp-2 cell line by IFA. 
      Seven sera which were ANA and anti-dsDNA positive with IFA were also found to be 
      positive with WB and their ANA patterns with the specific ANA WB bands were also 
      evaluated. It was observed that IFA results were in concordance with WB results. 
      Our data indicated that the above findings should be controlled and evaluated 
      with a more advanced method such as western blotting technique in order to 
      confirm the presence of specific antibodies along with clinical outcome of the 
      patients. As a result we think that ANA WB method is an appropriate technique in 
      diagnosis of CTD as anti-dsDNA and ANA bands can be evaluated together with this 
      method.
FAU - Yilmaz, Ozlem
AU  - Yilmaz O
AD  - Dokuz Eylul Universitesi Tip Fakultesi Mikrobiyoloji ve Klinik Mikrobiyoloji 
      Anabilim Dali, Inciralti, Izmir, Turkey.
FAU - Karaman, Meral
AU  - Karaman M
FAU - Ergon, M Cem
AU  - Ergon MC
FAU - Bahar, I Hakki
AU  - Bahar IH
FAU - Yulug, Nuran
AU  - Yulug N
LA  - tur
PT  - English Abstract
PT  - Journal Article
TT  - Konnektif doku hastaliklarinin tanisinda antinukleer (ANA) ve anti-double 
      stranded dna (anti-dsDNA) antikorlarinin onemi.
PL  - Turkey
TA  - Turkiye Parazitol Derg
JT  - Turkiye parazitolojii dergisi
JID - 9425544
EDAT- 2006/11/25 09:00
MHDA- 2006/11/25 09:01
CRDT- 2006/11/25 09:00
PHST- 2006/11/25 09:00 [pubmed]
PHST- 2006/11/25 09:01 [medline]
PHST- 2006/11/25 09:00 [entrez]
PST - ppublish
SO  - Turkiye Parazitol Derg. 2005;29(4):287-90.