PMID- 17125827
OWN - NLM
STAT- MEDLINE
DCOM- 20070312
LR  - 20131121
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 119
IP  - 2
DP  - 2007 Feb
TI  - Differentiation of monocytes into macrophages induces the upregulation of 
      histamine H1 receptor.
PG  - 472-81
AB  - BACKGROUND: Histamine modulates several functions in human monocytes, 
      macrophages, and dendritic cells. However, responses elicited by histamine differ 
      depending on cell type, suggesting variable expression of histamine receptors in 
      the monocyte/macrophage lineage. OBJECTIVE: We sought to examine whether the 
      expression of H(1) receptors was regulated by cell differentiation of human 
      monocytes into macrophages or dendritic cells. METHODS: Expression of H(1) 
      receptor was evaluated by RT-PCR and western blot in monocytes, monocyte-derived 
      macrophages (MDMs), monocyte-derived dendritic cells (DCs) and human lung 
      macrophages (HLMs). RESULTS: Expression of H(1) receptor mRNA and protein was 
      higher in HLMs and DCs than in monocytes. H(1) expression was approximately 
      15-fold and 4-fold higher in MDMs and HLMs, respectively, as compared with that 
      seen in monocytes. H(1) receptor protein was undetectable in monocytes, whereas 
      it was conspicuous in MDMs. Simultaneous analysis of H(2) and H(1) mRNA 
      expression indicated that the H(2)/H(1) ratio decreased from 202.7 +/- 14.8 in 
      monocytes to 2.2 +/- 0.4 in MDM and 39.5 +/- 5.0 in DCs. Incubation of monocytes 
      with histamine neither affected intracellular Ca(2+) concentrations nor 
      influenced IL-8 production. In contrast, histamine rapidly induced a Ca(2+) 
      signal and stimulated IL-8 production in MDMs. Both effects were inhibited by 
      H(1) blockade with levocetirizine, but not by H(2) blockade with ranitidine. 
      CONCLUSIONS: Differentiation of monocytes into macrophages or dendritic cells is 
      associated with profound changes of histamine receptor expression. Upregulation 
      of H(1) receptors confers on macrophages the capacity of being activated by 
      histamine. CLINICAL IMPLICATIONS: Regulation of H(1) and H(2) receptor expression 
      in the monocyte/macrophage lineage can be relevant to the pathogenesis of 
      allergic inflammation.
FAU - Triggiani, Massimo
AU  - Triggiani M
AD  - Division of Clinical Immunology and Allergy, University of Naples Federico II, 
      Naples, Italy. triggian@unina.it <triggian@unina.it>
FAU - Petraroli, Angelica
AU  - Petraroli A
FAU - Loffredo, Stefania
AU  - Loffredo S
FAU - Frattini, Annunziata
AU  - Frattini A
FAU - Granata, Francescopaolo
AU  - Granata F
FAU - Morabito, Paolo
AU  - Morabito P
FAU - Staiano, Rosaria Ilaria
AU  - Staiano RI
FAU - Secondo, Agnese
AU  - Secondo A
FAU - Annunziato, Lucio
AU  - Annunziato L
FAU - Marone, Gianni
AU  - Marone G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061127
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Histamine H1)
RN  - 0 (Receptors, Histamine H2)
RN  - 820484N8I3 (Histamine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Cell Differentiation
MH  - Gene Expression Regulation
MH  - Histamine/pharmacology
MH  - Humans
MH  - Interleukin-6/biosynthesis
MH  - Macrophages/*metabolism
MH  - Monocytes/*cytology/metabolism
MH  - RNA, Messenger/analysis
MH  - Receptors, Histamine H1/analysis/*genetics
MH  - Receptors, Histamine H2/genetics
MH  - Up-Regulation
EDAT- 2006/11/28 09:00
MHDA- 2007/03/14 09:00
CRDT- 2006/11/28 09:00
PHST- 2006/02/15 00:00 [received]
PHST- 2006/07/26 00:00 [revised]
PHST- 2006/09/22 00:00 [accepted]
PHST- 2006/11/28 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/11/28 09:00 [entrez]
AID - S0091-6749(06)02002-1 [pii]
AID - 10.1016/j.jaci.2006.09.027 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2007 Feb;119(2):472-81. doi: 10.1016/j.jaci.2006.09.027. 
      Epub 2006 Nov 27.