PMID- 17127000
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20201209
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 412
IP  - 1
DP  - 2007 Jan 22
TI  - Early rapid rise in EAAT2 expression follows the period of maximal seizure 
      susceptibility in human brain.
PG  - 89-94
AB  - Seizures are relatively common in the first weeks of life and can have lasting 
      effects on brain development due to glutamate excitotoxicity. The excitatory 
      amino acid transporter 2 (EAAT2) is responsible for the majority of glutamate 
      uptake in the brain and mice with this gene deleted die from seizures. Therefore, 
      we reasoned that developmental changes in the expression of EAAT2 might correlate 
      with the period of increased susceptibility to seizures in humans, reflecting a 
      changing vulnerability to excitotoxic insults. Expression levels of eight splice 
      forms of EAAT2 were measured using quantitative RT-PCR from human prefrontal 
      cortex and hippocampus at 1-2 months, 1-2 years, 8 years, 15-16 years, and 18-22 
      years of age. There was a significant increase in expression of most isoforms 
      between 1-2 months and 1-2 years with isoform-specific patterns after that 
      period. The increase in EAAT2 expression during the first 2 years of life 
      corresponds to a period of maximal synapse formation and other changes in the 
      glutamatergic system such as increased NMDA receptor expression. Moreover, the 
      low expression of EAAT2 in the first months of life corresponds to the period of 
      maximum susceptibility to seizures.
FAU - Lauriat, Tara L
AU  - Lauriat TL
AD  - Department of Psychiatry, Mount Sinai School of Medicine, One Gustave L. Levy 
      Place, New York, NY 10029, USA.
FAU - Schmeidler, James
AU  - Schmeidler J
FAU - McInnes, L Alison
AU  - McInnes LA
LA  - eng
GR  - N01-HD-4-3368/HD/NICHD NIH HHS/United States
GR  - N01-HD-4-3383/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061128
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Glutamate Plasma Membrane Transport Proteins)
RN  - 0 (SLC1A2 protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Analysis of Variance
MH  - Child
MH  - Excitatory Amino Acid Transporter 2
MH  - Exons
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Glutamate Plasma Membrane Transport Proteins/*metabolism
MH  - Hippocampus/growth & development/*metabolism
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Postmortem Changes
MH  - Prefrontal Cortex/growth & development/*metabolism
MH  - Seizures/*pathology
EDAT- 2006/11/28 09:00
MHDA- 2007/03/28 09:00
CRDT- 2006/11/28 09:00
PHST- 2006/08/08 00:00 [received]
PHST- 2006/10/19 00:00 [revised]
PHST- 2006/10/24 00:00 [accepted]
PHST- 2006/11/28 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2006/11/28 09:00 [entrez]
AID - S0304-3940(06)01148-7 [pii]
AID - 10.1016/j.neulet.2006.10.037 [doi]
PST - ppublish
SO  - Neurosci Lett. 2007 Jan 22;412(1):89-94. doi: 10.1016/j.neulet.2006.10.037. Epub 
      2006 Nov 28.