PMID- 17127022 OWN - NLM STAT- MEDLINE DCOM- 20070727 LR - 20161124 IS - 0378-8741 (Print) IS - 0378-8741 (Linking) VI - 111 IP - 1 DP - 2007 Apr 20 TI - Protective activity of andrographolide and arabinogalactan proteins from Andrographis paniculata Nees. against ethanol-induced toxicity in mice. PG - 13-21 AB - To find out the active principles against ethanol-induced toxicity in mice, Andrographis paniculata Nees. (Ap) was chosen and isolated andrographolide (ANDRO) and arabinogalactan proteins (AGPs). ANDRO was detected by HPTLC, FTIR and quantified by HPLC (10mg/g of Ap powder). AGPs was detected by beta-glucosyl Yariv staining of SDS-PAGE gel, FTIR and quantified by single radial gel diffusion assay with beta-glucosyl Yariv reagent (0.5mg/g Ap powder). The mice are pretreated intra-peritoneally (i.p.) with different doses (62.5, 125, 250, and 500mg/kg) of body weight of mice] of ANDRO and AGPs for 7 days and then ethanol (7.5g/kg of body weight) was injected, i.p. Besides, silymarin was used as standard hepatoprotective agent for comparative study with ANDRO and AGPs. The ameliorative activity of ANDRO and AGP against hepatic renal alcohol toxicity was measured by assessing GOT, GPT, ACP, ALP and LP levels in liver and kidney. It has been observed that pretreatment of mice with ANDRO and AGPs at 500mg/kg of body weight and 125mg/kg of body weight respectively could able to minimize the toxicity in compare to ethanol treated group as revealed by the different enzymatic assay in liver and kidney tissues and the results were comparable with silymarin. Hence, out of several ill-defined compounds present in Ap, ANDRO and AGPs are the potential bioactive compounds responsible for protection against ethanol-induced toxicity. FAU - Singha, Prajjal K AU - Singha PK AD - Microbiology and Immunology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, West Bengal, India. FAU - Roy, Somenath AU - Roy S FAU - Dey, Satyahari AU - Dey S LA - eng PT - Comparative Study PT - Journal Article DEP - 20061028 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Central Nervous System Depressants) RN - 0 (Diterpenes) RN - 0 (Galactans) RN - 0 (Glucosides) RN - 0 (Protective Agents) RN - 0 (Silymarin) RN - 0 (Yariv reagent) RN - 3K9958V90M (Ethanol) RN - 410105JHGR (andrographolide) RN - DHD7FFG6YS (Phloroglucinol) RN - EC 2.6.1.1 (Aspartate Aminotransferases) RN - EC 3.1.3.1 (Alkaline Phosphatase) RN - EC 3.1.3.2 (Acid Phosphatase) RN - SL4SX1O487 (arabinogalactan) SB - IM MH - Acid Phosphatase/metabolism MH - Alkaline Phosphatase/metabolism MH - *Andrographis/chemistry MH - Animals MH - Aspartate Aminotransferases/metabolism MH - Central Nervous System Depressants MH - Chromatography, High Pressure Liquid MH - Disease Models, Animal MH - Diterpenes/isolation & purification/*pharmacology MH - Dose-Response Relationship, Drug MH - Electrophoresis, Polyacrylamide Gel MH - Ethanol MH - Galactans/isolation & purification/*pharmacology MH - Glucosides MH - India MH - Kidney Diseases/chemically induced/metabolism/*prevention & control MH - Lipid Peroxidation/drug effects MH - Liver Diseases, Alcoholic/etiology/metabolism/*prevention & control MH - Male MH - Mice MH - Phloroglucinol/analogs & derivatives MH - Protective Agents/isolation & purification/*pharmacology MH - Silymarin/pharmacology MH - Spectroscopy, Fourier Transform Infrared EDAT- 2006/11/28 09:00 MHDA- 2007/07/28 09:00 CRDT- 2006/11/28 09:00 PHST- 2005/07/26 00:00 [received] PHST- 2006/09/27 00:00 [revised] PHST- 2006/10/12 00:00 [accepted] PHST- 2006/11/28 09:00 [pubmed] PHST- 2007/07/28 09:00 [medline] PHST- 2006/11/28 09:00 [entrez] AID - S0378-8741(06)00554-X [pii] AID - 10.1016/j.jep.2006.10.026 [doi] PST - ppublish SO - J Ethnopharmacol. 2007 Apr 20;111(1):13-21. doi: 10.1016/j.jep.2006.10.026. Epub 2006 Oct 28.