PMID- 17127486
OWN - NLM
STAT- MEDLINE
DCOM- 20070202
LR  - 20161019
IS  - 0953-7104 (Print)
IS  - 0953-7104 (Linking)
VI  - 17
IP  - 8
DP  - 2006 Dec
TI  - Platelet and monocyte activation by hyperglycemia and hyperinsulinemia in healthy 
      subjects.
PG  - 577-85
AB  - Type 2 diabetes mellitus (T2DM) patients have hyperglycemia and hyperinsulinemia 
      and increased risk of atherosclerosis and acute vascular complications. We have 
      reported elevated circulating tissue factor procoagulant activity (TF-PCA) during 
      hyperglycemia (HG) and hyperinsulinemia (HI) in normal subjects. To evaluate the 
      effect of hyperglycemia and hyperinsulinemia on blood cell activation, we 
      assessed platelet CD40L and P-selectin, monocyte tissue factor (TF), and the 
      formation of monocyte-platelet and neutrophil-platelet aggregates. These were 
      assessed in the resting state and following activation with ADP and thrombin 
      (SFLLRN). Healthy individuals were subjected to 24 h of hyperglycemia and 
      hyperinsulinemia, selective hyperglycemia, selective hyperinsulinemia, or normal 
      glucose and insulin. Platelet CD40L expression increased with high glucose/high 
      insulin, selective hyperglycemia and selective hyperinsulinemia. 
      Monocyte-platelet aggregates increased with high glucose/high insulin. Monocyte 
      TF expression increased with high glucose/high insulin and with selective 
      hyperinsulinemia. Upon stimulation with ADP and SFLLRN, monocyte-platelet and 
      neutrophil-platelet aggregates, platelet CD40L and P-selectin, and monocyte TF 
      increased compared to the resting state but was not different between 0 and 24 h, 
      indicating that the responsiveness to those agonists was not altered. 
      CONCLUSIONS: Hyperglycemia-hyperinsulinemia in healthy individuals induced 
      platelet activation and monocyte TF expression promoting a procoagulant and 
      proinflammatory state that may contribute to acute vascular events and 
      atherogenesis. Platelet responsiveness to activation with ADP or SFLLRN appears 
      not to be altered by hyperglycemia-hyperinsulinemia.
FAU - Vaidyula, Vijender R
AU  - Vaidyula VR
AD  - Sol Sherry Thrombosis Research Center, Temple University School of Medicine, 
      Philadelphia, PA 19140, USA.
FAU - Boden, Guenther
AU  - Boden G
FAU - Rao, A Koneti
AU  - Rao AK
LA  - eng
GR  - R01 DK058895/DK/NIDDK NIH HHS/United States
GR  - R01-DK58895/DK/NIDDK NIH HHS/United States
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Blood Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (thrombin receptor peptide (42-47))
RN  - 61D2G4IYVH (Adenosine Diphosphate)
SB  - IM
MH  - Adenosine Diphosphate/pharmacology
MH  - Adult
MH  - Atherosclerosis/blood/etiology
MH  - Blood Platelets/*metabolism
MH  - Blood Proteins/analysis
MH  - Diabetes Complications/blood
MH  - Diabetes Mellitus, Type 2/blood
MH  - Female
MH  - Humans
MH  - Hyperglycemia/*blood/etiology
MH  - Hyperinsulinism/*blood
MH  - Inflammation/blood/etiology
MH  - *Macrophage Activation/drug effects
MH  - Male
MH  - Monocytes/*metabolism
MH  - Peptide Fragments/pharmacology
MH  - *Platelet Aggregation/drug effects
EDAT- 2006/11/28 09:00
MHDA- 2007/02/03 09:00
CRDT- 2006/11/28 09:00
PHST- 2006/11/28 09:00 [pubmed]
PHST- 2007/02/03 09:00 [medline]
PHST- 2006/11/28 09:00 [entrez]
AID - X684U5855L334HP7 [pii]
AID - 10.1080/09537100600760814 [doi]
PST - ppublish
SO  - Platelets. 2006 Dec;17(8):577-85. doi: 10.1080/09537100600760814.