PMID- 17127633
OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR  - 20131213
IS  - 1369-3786 (Print)
IS  - 1369-3786 (Linking)
VI  - 44
IP  - 8
DP  - 2006 Dec
TI  - B1 cells contribution to susceptibility in experimental paracoccidioidomycosis: 
      immunoglobulin isotypes and repertoire determination.
PG  - 755-66
AB  - Paracoccidioidomycosis (PCM) is the most prevalent systemic mycosis in Latin 
      America. The experimental murine model has been used to approach the disease, 
      with susceptible and resistant mice strains that reproduce most of the main human 
      immunological features. Since the hypergammaglobulinemia observed in susceptible 
      mice and humans might have an influence on B1 cells, we investigated its role 
      during the experimental infection with Paracoccidiodes brasiliensis. CBA/Nxid 
      mice, deficient in B1 cells, and CBA/Nxid reconstituted with B1 cells isolated 
      from the non-mutant CBA/J strain were infected with 106 yeast forms of P. 
      brasiliensis. At the 8th and 22nd week post infection the DTH response of 
      CBA/Nxid mice was significantly higher after 24 h of P. brasiliensis antigens 
      inoculation and the specific humoral response was reduced, in comparison to CBA/J 
      or recCBA/Nxid. Production of NAbs is a hallmark of the B1 subset. Higher Ig 
      productions to auto antigens such as DNA, MBP and RBC were observed in CBA/J 
      infected mice or recCBA/Nxid. Anti P. brasiliensis IgG2a was produced by CBA/Nxid 
      mice early in infection, while CBA/J or recCBA/Nxid presented increased levels of 
      this isotype only after the 8th week of infection. Furthermore, western blotting 
      analysis showed that CBA/Nxid mice expanded less clones against P. brasiliensis 
      antigens, with weakly detectable anti-gp43 antibodies while CBA/J mice produce 
      IgM anti-gp43 at the 2nd week of infection and IgG anti-gp43 at the 2nd and 8th 
      week. On the other hand, recognition of gp70, a fungal antigen that, as gp43, 
      inhibits macrophage activation was not compromised in B1 deficient mice. These 
      results suggest that B1 cells might have influence in the kinetic of production 
      of protective isotypes of immunoglobulins and their repertoire that could 
      contribute to an early drive towards a Th2 response, affecting the cellular 
      response in susceptible mice during experimental paracoccidiodomycosis.
FAU - Marcelino Franca, Karla
AU  - Marcelino Franca K
AD  - Laboratorio de Biologia do Reconhecer, Centro de Biociencias e Biotecnologia, 
      Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil.
FAU - Vericimo, Mauricio A
AU  - Vericimo MA
FAU - Retamal, Claudio
AU  - Retamal C
FAU - Kipnis, Thereza L
AU  - Kipnis TL
FAU - Arnholdt, Andrea C V
AU  - Arnholdt AC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Med Mycol
JT  - Medical mycology
JID - 9815835
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin Isotypes)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/*blood
MH  - Antigens, Fungal/immunology
MH  - B-Lymphocyte Subsets/*immunology
MH  - Blotting, Western
MH  - Disease Models, Animal
MH  - *Disease Susceptibility
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fungal Proteins/immunology
MH  - Glycoproteins/immunology
MH  - Hypersensitivity, Delayed
MH  - Immunoglobulin G/blood
MH  - Immunoglobulin Isotypes/*blood
MH  - Immunoglobulin M/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology
EDAT- 2006/11/28 09:00
MHDA- 2007/04/05 09:00
CRDT- 2006/11/28 09:00
PHST- 2006/11/28 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2006/11/28 09:00 [entrez]
AID - M60212L627711772 [pii]
AID - 10.1080/13693780601009485 [doi]
PST - ppublish
SO  - Med Mycol. 2006 Dec;44(8):755-66. doi: 10.1080/13693780601009485.