PMID- 17127755
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20221207
IS  - 1470-1626 (Print)
IS  - 1470-1626 (Linking)
VI  - 132
IP  - 6
DP  - 2006 Dec
TI  - Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum 
      levels of CB-153 and p,p'-DDE in European and Inuit populations.
PG  - 949-58
AB  - Persistent organochlorine pollutants (POPs) are suspected to interfere with 
      hormone activity and the normal homeostasis of spermatogenesis. We investigated 
      the relationships between sperm DNA fragmentation, apoptotic markers identified 
      on ejaculated spermatozoa and POP levels in the blood of 652 adult males (200 
      Inuits from Greenland, 166 Swedish, 134 Polish and 152 Ukrainian). Serum levels 
      of 2, 2', 4, 4', 5, 5'-hexachlorobiphenyl (CB-153), as a proxy of the total POP 
      burden, and of 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p'-DDE), as a 
      proxy of the total DDT exposure were determined. Sperm DNA fragmentation was 
      measured by using the TUNEL assay, whereas immunofluorescence methods were 
      utilized for detecting pro-apoptotic (Fas) and anti-apoptotic (Bcl-xL) markers. 
      Both TUNEL assay and apoptotic markers were statistically differed across the 
      four populations. No correlation between neither sperm DNA fragmentation nor 
      apoptotic sperm parameters and the large variations in POPs exposure was observed 
      for the separate study groups. However, considering the European populations 
      taken together, we showed that both %TUNEL positivity and Bcl-xL were related to 
      CB-153 serum levels, whereas our study failed to demonstrate any relations 
      between DDE and %TUNEL positivity and apoptotic sperm biomarkers (Fas and Bcl-xL) 
      in any region or overall regions. These results suggest that CB-153 and related 
      chemicals might alter sperm DNA integrity and Bcl-xL levels in European adult 
      males, but not in the highly exposed Inuit men. Additional issues (genetic 
      background, lifestyle habits and characterization of total xeno-hormonal 
      activities) need to be investigated in order to fully assess the population 
      variations observed.
FAU - Stronati, A
AU  - Stronati A
AD  - Institute of Biology and Genetics, Marche Polytechnic University, Ancona, Italy.
FAU - Manicardi, G C
AU  - Manicardi GC
FAU - Cecati, M
AU  - Cecati M
FAU - Bordicchia, M
AU  - Bordicchia M
FAU - Ferrante, L
AU  - Ferrante L
FAU - Spano, M
AU  - Spano M
FAU - Toft, G
AU  - Toft G
FAU - Bonde, J P
AU  - Bonde JP
FAU - Jonsson, B A G
AU  - Jonsson BA
FAU - Rignell-Hydbom, A
AU  - Rignell-Hydbom A
FAU - Rylander, L
AU  - Rylander L
FAU - Giwercman, A
AU  - Giwercman A
FAU - Pedersen, H S
AU  - Pedersen HS
FAU - Bonefeld-Jorgensen, E C
AU  - Bonefeld-Jorgensen EC
FAU - Ludwicki, J K
AU  - Ludwicki JK
FAU - Lesovoy, V
AU  - Lesovoy V
FAU - Sakkas, D
AU  - Sakkas D
FAU - Bizzaro, D
AU  - Bizzaro D
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Biomarkers)
RN  - 0 (Environmental Pollutants)
RN  - 0 (bcl-X Protein)
RN  - 0 (fas Receptor)
RN  - 4M7FS82U08 (Dichlorodiphenyl Dichloroethylene)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - ZRU0C9E32O (2,4,5,2',4',5'-hexachlorobiphenyl)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Biomarkers/analysis
MH  - DNA Fragmentation
MH  - Dichlorodiphenyl Dichloroethylene/blood
MH  - Environmental Exposure
MH  - Environmental Pollutants/blood/*toxicity
MH  - Flow Cytometry
MH  - Greenland
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - *Inuit
MH  - Linear Models
MH  - Male
MH  - Poland
MH  - Polychlorinated Biphenyls/blood/*toxicity
MH  - Semen/chemistry
MH  - Sperm Count
MH  - Spermatozoa/drug effects/*pathology
MH  - Sweden
MH  - Ukraine
MH  - White People
MH  - bcl-X Protein/analysis
MH  - fas Receptor/analysis
EDAT- 2006/11/28 09:00
MHDA- 2007/06/20 09:00
CRDT- 2006/11/28 09:00
PHST- 2006/11/28 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2006/11/28 09:00 [entrez]
AID - 132/6/949 [pii]
AID - 10.1530/rep.1.01034 [doi]
PST - ppublish
SO  - Reproduction. 2006 Dec;132(6):949-58. doi: 10.1530/rep.1.01034.