PMID- 17129574
OWN - NLM
STAT- MEDLINE
DCOM- 20070305
LR  - 20191210
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 352
IP  - 2
DP  - 2007 Jan 12
TI  - Regulation of Ca2+ mobilization by prolactin in mammary gland cells: possible 
      role of secretory pathway Ca2+-ATPase type 2.
PG  - 537-42
AB  - Regulatory role of prolactin (PRL) on Ca2+ mobilization in human mammary gland 
      cell line MCF-7 was examined. Direct addition of PRL did not affect cytoplasmic 
      Ca2+ concentration ([Ca2+]i); however, treatment with PRL for 24h significantly 
      decreased the peak level and duration time of [Ca2+]i elevation evoked by ATP or 
      thapsigargin (TG). Intracellular Ca2+ release by IP3 or TG in permeablized cells 
      was not decreased after PRL-treatment, indicating that the Ca2+ release was not 
      impaired by PRL treatment. Extracellular Ca2+ entry evoked by ATP or TG was 
      likely to be intact, because entry of extracellular Ba2+ was not affected by PRL 
      treatment. Among Ca2+-ATPases expressed in MCF-7 cells, we found significant 
      increase of secretory pathway Ca2+-ATPase type 2 (SPCA2) mRNA in PRL-treated 
      cells by RT-PCR experiments including quantitative RT-PCR. Knockdown of SPCA2 by 
      siRNA in PRL-treated cells showed similar Ca2+ mobilization to that in 
      PRL-untreated cells. The present results suggest that PRL facilitates Ca2+ 
      transport into Golgi apparatus and may contribute the supply of Ca2+ to milk.
FAU - Anantamongkol, Utchariya
AU  - Anantamongkol U
AD  - Department of Physiology, Faculty of Sciences, Mahidol University, Rama VI Road, 
      Bangkok 10400, Thailand.
FAU - Takemura, Haruo
AU  - Takemura H
FAU - Suthiphongchai, Tuangporn
AU  - Suthiphongchai T
FAU - Krishnamra, Nateetip
AU  - Krishnamra N
FAU - Horio, Yoshiyuki
AU  - Horio Y
LA  - eng
PT  - Journal Article
DEP - 20061120
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 9002-62-4 (Prolactin)
RN  - EC 7.2.2.10 (ATP2C2 protein, human)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Breast Neoplasms/*metabolism
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*drug effects
MH  - Calcium-Transporting ATPases/*metabolism
MH  - Cell Line, Tumor
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Mammary Glands, Human/*metabolism
MH  - Prolactin/*administration & dosage
EDAT- 2006/11/30 09:00
MHDA- 2007/03/06 09:00
CRDT- 2006/11/30 09:00
PHST- 2006/11/09 00:00 [received]
PHST- 2006/11/13 00:00 [accepted]
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/03/06 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
AID - S0006-291X(06)02527-7 [pii]
AID - 10.1016/j.bbrc.2006.11.055 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Jan 12;352(2):537-42. doi: 
      10.1016/j.bbrc.2006.11.055. Epub 2006 Nov 20.