PMID- 17130301
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 80
IP  - 24
DP  - 2006 Dec
TI  - Screening random peptide libraries with subacute sclerosing panencephalitis 
      brain-derived recombinant antibodies identifies multiple epitopes in the 
      C-terminal region of the measles virus nucleocapsid protein.
PG  - 12121-30
AB  - Infectious and inflammatory diseases of the CNS are often characterized by a 
      robust B-cell response that manifests as increased intrathecal immunoglobulin G 
      (IgG) synthesis and the presence of oligoclonal bands. We previously used laser 
      capture microdissection and single-cell PCR to analyze the IgG variable regions 
      of plasma cells from the brain of a patient with subacute sclerosing 
      panencephalitis (SSPE). Five of eight human IgG1 recombinant antibodies (rAbs) 
      derived from SSPE brain plasma cell clones recognized the measles virus (MV) 
      nucleocapsid protein, confirming that the antibody response in SSPE targets 
      primarily the agent causing disease. In this study, as part of our work on 
      antigen identification, we used four rAbs to probe a random phage-displayed 
      peptide library to determine if epitopes within the MV nucleocapsid protein could 
      be identified with SSPE brain rAbs. All four of the SSPE rAbs enriched 
      phage-displayed peptide sequences that reacted specifically to their panning rAb 
      by enzyme-linked immunosorbent assay. BLASTP searches of the NCBI protein 
      database revealed clear homologies in three peptides and different amino acid 
      stretches within the 65 C-terminal amino acids of the MV nucleocapsid protein. 
      The specificities of SSPE rAbs to these regions of the MV nucleocapsid protein 
      were confirmed by binding to synthetic peptides or to short cDNA expression 
      products. These results indicate the feasibility of using peptide screening for 
      antigen discovery in central nervous system inflammatory diseases of unknown 
      etiology, such as multiple sclerosis, neurosarcoidosis, or Behcet's syndrome.
FAU - Owens, Gregory P
AU  - Owens GP
AD  - Department of Neurology, University of Colorado Health Sciences Center, 4200 E. 
      9th Avenue, Mail Stop B182, Denver, CO 80262, USA.
FAU - Shearer, Andrew J
AU  - Shearer AJ
FAU - Yu, Xiaoli
AU  - Yu X
FAU - Ritchie, Alanna M
AU  - Ritchie AM
FAU - Keays, Kathryne M
AU  - Keays KM
FAU - Bennett, Jeffrey L
AU  - Bennett JL
FAU - Gilden, Donald H
AU  - Gilden DH
FAU - Burgoon, Mark P
AU  - Burgoon MP
LA  - eng
GR  - P01 NS032623/NS/NINDS NIH HHS/United States
GR  - R01 NS041549/NS/NINDS NIH HHS/United States
GR  - NS 32623/NS/NINDS NIH HHS/United States
GR  - NS 41549/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antibodies, Viral)
RN  - 0 (Epitopes)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Peptide Library)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antibodies, Viral/*genetics
MH  - Computational Biology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/*genetics
MH  - Humans
MH  - Measles virus/*genetics
MH  - Molecular Sequence Data
MH  - Nucleocapsid Proteins/*genetics
MH  - Peptide Library
MH  - Peptides/*genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Subacute Sclerosing Panencephalitis/*immunology
PMC - PMC1676253
EDAT- 2006/11/30 09:00
MHDA- 2007/02/21 09:00
PMCR- 2007/04/01
CRDT- 2006/11/30 09:00
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
PHST- 2007/04/01 00:00 [pmc-release]
AID - 80/24/12121 [pii]
AID - 1704-06 [pii]
AID - 10.1128/JVI.01704-06 [doi]
PST - ppublish
SO  - J Virol. 2006 Dec;80(24):12121-30. doi: 10.1128/JVI.01704-06.