PMID- 17133037
OWN - NLM
STAT- MEDLINE
DCOM- 20070215
LR  - 20191210
IS  - 0195-7910 (Print)
IS  - 0195-7910 (Linking)
VI  - 27
IP  - 4
DP  - 2006 Dec
TI  - Highly sensitive HLA-DNA typing from formalin-fixed and paraffin-embedded tissue 
      samples.
PG  - 347-51
AB  - Nucleotide sequences have been determined for more than 1700 different alleles at 
      the core of the human leukocyte antigen (HLA) system. The highly polymorphic 
      character of these genes affects adaptive immune response and is also useful for 
      forensic applications. HLA typing from formalin-fixed and paraffin-embedded 
      tissue provides abundant useful information for both clinical settings and 
      forensic investigations. This study, which investigated the potential use of DNA 
      from formalin-fixed and paraffin-embedded tissue samples in an HLA PCR 
      sequence-specific primer and probe (SPP) system, showed that tissue fixed in 
      formalin for less than 3 days and embedded in paraffin can serve as a useful 
      source of DNA for PCR-SPP typing kits.
FAU - Ota, Masao
AU  - Ota M
AD  - Department of Legal Medicine, Shinshu University School of Medicine, Asahi, 
      Matsumoto, Japan. otamasao@sch.md.shinshu-u.ac.jp
FAU - Shimada, Kazunori
AU  - Shimada K
FAU - Asamura, Hideki
AU  - Asamura H
FAU - Katsuyama, Yoshihiko
AU  - Katsuyama Y
FAU - Fukushima, Hirofumi
AU  - Fukushima H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Forensic Med Pathol
JT  - The American journal of forensic medicine and pathology
JID - 8108948
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
RN  - 1HG84L3525 (Formaldehyde)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Autopsy
MH  - DNA/*analysis
MH  - DNA Primers
MH  - Forensic Medicine
MH  - Formaldehyde
MH  - HLA Antigens/*analysis
MH  - Humans
MH  - Paraffin Embedding
MH  - Polymerase Chain Reaction/*methods
MH  - Predictive Value of Tests
MH  - Sensitivity and Specificity
EDAT- 2006/11/30 09:00
MHDA- 2007/02/16 09:00
CRDT- 2006/11/30 09:00
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/02/16 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
AID - 00000433-200612000-00017 [pii]
AID - 10.1097/01.paf.0000233558.79449.0c [doi]
PST - ppublish
SO  - Am J Forensic Med Pathol. 2006 Dec;27(4):347-51. doi: 
      10.1097/01.paf.0000233558.79449.0c.