PMID- 17133548
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20161124
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 79
IP  - 1
DP  - 2007 Jan
TI  - The prevalence of chromosomally integrated human herpesvirus 6 genomes in the 
      blood of UK blood donors.
PG  - 45-51
AB  - A lesser-recognized form of human herpesvirus 6 (HHV-6) persistence is 
      integration of the viral genome in a host chromosome and high viral copy numbers 
      in blood or sera are characteristic of this phenomenon. A cross-sectional study 
      was performed to determine the frequency of high HHV-6 viral loads in whole blood 
      (>6 log(10) copies/ml) in a population of blood donors in London, UK. Blood 
      samples from 500 anonymized blood donors were collected from one donation center, 
      DNA extracted, and quantitative realtime PCR used to measure viral load. Four 
      samples (0.8%) were found to have high viral copy numbers of HHV-6 (median 6.7 
      log(10) copies/ml; range 6.5- 6.9 log(10) copies/ml). Cellular DNA was also 
      quantitated using qRT-PCR for beta-globin. By comparing these two results, we 
      calculated that there were between two and five copies of HHV-6 present per cell 
      in these four donors. The median viral load detected in plasma from the four 
      individuals was 3.8 log(10) copies/ml (range 3.5-4.0 log(10) copies/ml). All 
      samples were HHV-6 variant B. In addition, a retrospective analysis of all 
      diagnostic blood samples performed for HHV-6 in our center showed a prevalence of 
      2.9% of high viral loads characteristic of integration. In conclusion, high viral 
      copy numbers of HHV-6, representing a population of viral integration, is 
      detected in 0.8% of UK blood donors. The presence of high HHV-6 viral loads in 
      healthy normal individuals reiterates the need to consider the confounding effect 
      of HHV-6 viral integration in any laboratory diagnosis of HHV-6 infection.
FAU - Leong, Hoe Nam
AU  - Leong HN
AD  - Division of Infection and Immunity, Centre for Virology, Hampstead Campus, Royal 
      Free and University College Medical School, London, UK.
FAU - Tuke, Philip W
AU  - Tuke PW
FAU - Tedder, Richard S
AU  - Tedder RS
FAU - Khanom, Aysha Begum
AU  - Khanom AB
FAU - Eglin, Roger P
AU  - Eglin RP
FAU - Atkinson, Claire E
AU  - Atkinson CE
FAU - Ward, Katherine N
AU  - Ward KN
FAU - Griffiths, Paul D
AU  - Griffiths PD
FAU - Clark, Duncan A
AU  - Clark DA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Blood Donors
MH  - DNA, Viral/*blood
MH  - Herpesviridae Infections/blood/*epidemiology/immunology/virology
MH  - Herpesvirus 6, Human/genetics/immunology/*isolation & purification
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - United Kingdom/epidemiology
MH  - Viral Load
MH  - *Virus Integration
EDAT- 2006/11/30 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/11/30 09:00
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
AID - 10.1002/jmv.20760 [doi]
PST - ppublish
SO  - J Med Virol. 2007 Jan;79(1):45-51. doi: 10.1002/jmv.20760.