PMID- 17133549
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20061204
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 79
IP  - 1
DP  - 2007 Jan
TI  - Human herpesvirus 8 genoprevalence in populations at disparate risks of Kaposi's 
      sarcoma.
PG  - 52-9
AB  - The prevalence of human herpesvirus 8 (HHV-8) in populations at different risks 
      of developing Kaposi's sarcoma (KS) was assessed using a protocol involving 
      immunomagnetic fractionation of CD45+ blood cells prior to detection of the HHV-8 
      genome by nested PCR. Preliminary studies using blood of eight gay men infected 
      by human immunodeficiency virus-1 (HIV-1) revealed that, for the detection of 
      HHV-8 DNA derived from open reading frame (ORF) 26 of the HHV-8 genome, this 
      protocol provided substantially higher rates (100%) compared to one involving red 
      blood cell (RBC) lysis (0%) and to another requiring double density gradient 
      centrifugation (DDGC) of leukocytes (13%). When the CD45+ fractionation protocol 
      was applied to samples from 103 other HIV-1-infected patients (the vast majority 
      of whom were gay men) and 100 blood donors, the ORF 26 DNA detection rates 
      obtained were 37% and 8%, respectively. When DNA from the variable region 1 of 
      ORF K1 was additionally amplified from samples of the blood donors, a detection 
      rate of 9% was achieved. This rate was highly concordant with the ORF 26 DNA 
      detection rate. Furthermore, the ORF K1 sequences were predominantly unique, 
      assignable to genotypes A1, A4, and C3. When assays for anti-HHV-8 and 
      anti-herpes simplex viruses (HSV) 1 and 2 were applied, significant concordances 
      between HHV-8 DNA detection rates and those relating to anti-HHV-8 and to 
      anti-HSV 1 and 2 were more frequently observed for HIV-1-infected patients than 
      for blood donors. The higher-than-expected HHV-8 genoprevalence rate in blood 
      donors requires further confirmation in view of its implications for 
      post-transfusion HHV-8 transmission.
FAU - Kumar, Navdeep
AU  - Kumar N
AD  - Oral Medicine and Special Needs, UCL Eastman Dental Institute, University College 
      London, University of London, London, UK. n.kumar@eastman.ucl.ac.uk
FAU - McLean, Ken
AU  - McLean K
FAU - Inoue, Naoki
AU  - Inoue N
FAU - Moles, David R
AU  - Moles DR
FAU - Scully, Crispian
AU  - Scully C
FAU - Porter, Stephen R
AU  - Porter SR
FAU - Teo, Chong Gee
AU  - Teo CG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Acquired Immunodeficiency Syndrome/blood/*virology
MH  - DNA, Viral/genetics
MH  - Herpesvirus 8, Human/genetics/*isolation & purification
MH  - Humans
MH  - Prevalence
MH  - Regression Analysis
MH  - Sarcoma, Kaposi/blood/*epidemiology/pathology/*virology
EDAT- 2006/11/30 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/11/30 09:00
PHST- 2006/11/30 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/11/30 09:00 [entrez]
AID - 10.1002/jmv.20728 [doi]
PST - ppublish
SO  - J Med Virol. 2007 Jan;79(1):52-9. doi: 10.1002/jmv.20728.