PMID- 17134735
OWN - NLM
STAT- MEDLINE
DCOM- 20070308
LR  - 20191210
IS  - 0046-8177 (Print)
IS  - 0046-8177 (Linking)
VI  - 38
IP  - 2
DP  - 2007 Feb
TI  - Interphase fluorescence in situ hybridization is more sensitive than BIOMED-2 
      polymerase chain reaction protocol in detecting IGH-BCL2 rearrangement in both 
      fixed and frozen lymph node with follicular lymphoma.
PG  - 365-72
AB  - The detection of t(14;18)(q32;q21) is advisable for the diagnosis of follicular 
      lymphoma (FL). In 51 patients with FL, we evaluated the applicability and 
      sensitivity of interphase fluorescence in situ hybridization (FISH) and 
      polymerase chain reaction (PCR) using commercially available reagents. In 23 
      patients, only a formalin-fixed lymph node was available. In 28 patients, both 
      frozen and formalin-fixed lymph nodes were evaluated. Fluorescence in situ 
      hybridization was found to be 100% applicable whatever the material type. With 
      the use of IGH-BCL2 dual-fusion, dual-color probes, t(14;18) translocation was 
      detected in 47 (92%) of 51 FL cases with concordant results between isolated 
      nuclei (n = 41) and frozen cytologic imprints (n = 28). Twenty-two 
      IGH-BCL2-positive cases were also studied on fixed sections with BCL2 split 
      signal probes showing a BCL2 split in all. Conversely, no BCL2 split was observed 
      in IGH-BCL2-negative cases (n = 4). Owing to DNA degradation as assessed by the 
      failure of control genes amplification, the applicability of PCR was found to be 
      76% in fixed lymph nodes (n = 51). After exclusion of the 12 noninformative 
      cases, the BIOMED-2 protocol allowed the detection of an IGH-BCL2 fusion in 25 
      (64%) of 39 fixed specimens with 11 PCR-negative (31%) of 36 FISH-positive cases. 
      Even on frozen material with 100% applicability, the amplification of a BCL2-JH 
      breakpoint was achieved in only 20 (71%) of 28 cases with 5 PCR-negative (20%) 
      out of 25 FISH-positive cases. Therefore, FISH was found superior to PCR (using 
      BIOMED-2 protocol) in detecting IGH-BCL2 fusion. Finally, FISH individualized 4 
      IGH-BCL2-negative FL cases without specific histopathologic features. With the 
      use of split signal DNA probes, 1 case showed a trisomy of the BCL2 locus and 
      another displayed BCL6 and IGH breakpoints that would suggest a t(3;14). Whether 
      such IGH-BCL2-negative cases are characterized by alternative oncogenetic 
      pathways remains to be determined.
FAU - Belaud-Rotureau, Marc-Antoine
AU  - Belaud-Rotureau MA
AD  - Pathology Laboratory, CHU Bordeaux, 33604 Pessac, France.
FAU - Parrens, Marie
AU  - Parrens M
FAU - Carrere, Nathalie
AU  - Carrere N
FAU - Turmo, Michele
AU  - Turmo M
FAU - Ferrer, Jackie
AU  - Ferrer J
FAU - de Mascarel, Antoine
AU  - de Mascarel A
FAU - Dubus, Pierre
AU  - Dubus P
FAU - Merlio, Jean-Philippe
AU  - Merlio JP
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061128
PL  - United States
TA  - Hum Pathol
JT  - Human pathology
JID - 9421547
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
SB  - IM
MH  - Chromosomes, Human, Pair 14
MH  - Chromosomes, Human, Pair 18
MH  - Frozen Sections
MH  - Humans
MH  - Immunoglobulin Heavy Chains/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase/genetics
MH  - Lymph Nodes/metabolism/*pathology
MH  - Lymphoma, Follicular/*diagnosis/genetics
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Polymerase Chain Reaction/*methods
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tissue Fixation
MH  - Translocation, Genetic
EDAT- 2006/12/01 09:00
MHDA- 2007/03/09 09:00
CRDT- 2006/12/01 09:00
PHST- 2006/07/12 00:00 [received]
PHST- 2006/08/22 00:00 [revised]
PHST- 2006/08/23 00:00 [accepted]
PHST- 2006/12/01 09:00 [pubmed]
PHST- 2007/03/09 09:00 [medline]
PHST- 2006/12/01 09:00 [entrez]
AID - S0046-8177(06)00531-4 [pii]
AID - 10.1016/j.humpath.2006.08.022 [doi]
PST - ppublish
SO  - Hum Pathol. 2007 Feb;38(2):365-72. doi: 10.1016/j.humpath.2006.08.022. Epub 2006 
      Nov 28.