PMID- 1713509
OWN - NLM
STAT- MEDLINE
DCOM- 19910904
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 78
IP  - 3
DP  - 1991 Aug 1
TI  - Potentiation of early hematopoiesis by tumor necrosis factor-alpha is followed by 
      inhibition of granulopoietic differentiation and proliferation.
PG  - 635-44
AB  - We have previously shown that tumor necrosis factor-alpha (TNF alpha) strongly 
      potentiates interleukin-3 (IL-3)-induced short-term proliferation of human CD34+ 
      hematopoietic progenitor cells (HPC). Using longer term cultures of CD34+ HPC, we 
      demonstrate here that this initial potentiation ceases after 10 to 12 days; 
      whereupon TNF alpha displays inhibitory effects. Thus, TNF alpha was found to 
      inhibit cells of granulocytic affiliation while it potentiates the development of 
      maturing cells of the monocytic lineage both in liquid and semi-solid (day 14 
      colony-forming unit) cultures. TNF alpha was demonstrated to reversibly block 
      granulocytic differentiation at the level of uncommitted CD13-, CD15- blast cells 
      that accumulate in IL-3 + TNF alpha cultures. Furthermore, growth of committed 
      granulocytes (CD15+) from IL-3 cultures was also inhibited by TNF alpha through 
      an arrest of cell cycle in G0/G1. Finally, the use of neutralizing anti-TNF alpha 
      monoclonal antibody and limiting dilution studies indicate that the inhibitory 
      effects of TNF alpha are direct. Taken together, our data demonstrate that, 
      following a phase of potentiation of proliferation of early HPC, TNF alpha 
      displays direct inhibitory effects due to negative interference with both 
      granulocytic differentiation and proliferation of granulocytic cells.
FAU - Caux, C
AU  - Caux C
AD  - Laboratory for Immunological Research, Schering-Plough, Dardilly, France.
FAU - Favre, C
AU  - Favre C
FAU - Saeland, S
AU  - Saeland S
FAU - Duvert, V
AU  - Duvert V
FAU - Durand, I
AU  - Durand I
FAU - Mannoni, P
AU  - Mannoni P
FAU - Banchereau, J
AU  - Banchereau J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Interleukin-3)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antigens, CD/analysis
MH  - Antigens, CD34
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Female
MH  - Fetal Blood
MH  - Granulocytes/*cytology/drug effects
MH  - Hematopoiesis/*drug effects
MH  - Humans
MH  - Infant, Newborn
MH  - Interleukin-3/*pharmacology
MH  - Kinetics
MH  - Phenotype
MH  - Pregnancy
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/08/01 00:00
MHDA- 1991/08/01 00:01
CRDT- 1991/08/01 00:00
PHST- 1991/08/01 00:00 [pubmed]
PHST- 1991/08/01 00:01 [medline]
PHST- 1991/08/01 00:00 [entrez]
AID - S0006-4971(20)85231-8 [pii]
PST - ppublish
SO  - Blood. 1991 Aug 1;78(3):635-44.