PMID- 17135314
OWN - NLM
STAT- MEDLINE
DCOM- 20070327
LR  - 20240413
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 5
DP  - 2007 Mar
TI  - The human TRIM5alpha restriction factor mediates accelerated uncoating of the 
      N-tropic murine leukemia virus capsid.
PG  - 2138-48
AB  - The host cell factors TRIM5alpha(hu) and Fv-1 restrict N-tropic murine leukemia 
      virus (N-MLV) infection at an early postentry step before or after reverse 
      transcription, respectively. Interestingly, the identity of residue 110 of the 
      MLV capsid determines susceptibility to both TRIM5alpha(hu) and Fv-1. In this 
      study, we investigate the fate of the MLV capsid in cells expressing either the 
      TRIM5alpha(hu) or Fv-1 restriction factor. The expression of TRIM5alpha(hu), but 
      not Fv-1, specifically promoted the premature conversion of particulate N-MLV 
      capsids within infected cells to soluble capsid proteins. The 
      TRIM5alpha(hu)-mediated disassembly of particulate N-MLV capsids was dependent 
      upon residue 110 of the viral capsid. Furthermore, the deletion or disruption of 
      TRIM5alpha(hu) domains necessary for potent N-MLV restriction completely 
      abrogated the disappearance of particulate N-MLV capsids observed with wild-type 
      TRIM5alpha(hu). These results suggest that premature disassembly of the viral 
      capsid contributes to the restriction of N-MLV infection by TRIM5alpha(hu), but 
      not by Fv-1.
FAU - Perron, Michel J
AU  - Perron MJ
AD  - Dana-Farber Cancer Institute, 44 Binney Street, JFB 824, Boston, MA 02115, USA.
FAU - Stremlau, Matthew
AU  - Stremlau M
FAU - Lee, Mark
AU  - Lee M
FAU - Javanbakht, Hassan
AU  - Javanbakht H
FAU - Song, Byeongwoon
AU  - Song B
FAU - Sodroski, Joseph
AU  - Sodroski J
LA  - eng
GR  - P30 AI060354/AI/NIAID NIH HHS/United States
GR  - R01 AI063987/AI/NIAID NIH HHS/United States
GR  - AI 063987/AI/NIAID NIH HHS/United States
GR  - AI 60354/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20061129
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antiviral Restriction Factors)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Fv1 protein, mouse)
RN  - 0 (Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM5 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Substitution
MH  - Animals
MH  - Antiviral Restriction Factors
MH  - Base Sequence
MH  - Capsid/physiology
MH  - Carrier Proteins/chemistry/genetics/*physiology
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - Humans
MH  - Leukemia Virus, Murine/genetics/pathogenicity/*physiology
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - NIH 3T3 Cells
MH  - Protein Structure, Tertiary
MH  - Proteins/genetics/physiology
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases
PMC - PMC1865943
EDAT- 2006/12/01 09:00
MHDA- 2007/03/28 09:00
PMCR- 2007/07/01
CRDT- 2006/12/01 09:00
PHST- 2006/12/01 09:00 [pubmed]
PHST- 2007/03/28 09:00 [medline]
PHST- 2006/12/01 09:00 [entrez]
PHST- 2007/07/01 00:00 [pmc-release]
AID - JVI.02318-06 [pii]
AID - 2318-06 [pii]
AID - 10.1128/JVI.02318-06 [doi]
PST - ppublish
SO  - J Virol. 2007 Mar;81(5):2138-48. doi: 10.1128/JVI.02318-06. Epub 2006 Nov 29.