PMID- 17135452
OWN - NLM
STAT- MEDLINE
DCOM- 20070427
LR  - 20181113
IS  - 1556-6811 (Print)
IS  - 1556-679X (Electronic)
IS  - 1556-679X (Linking)
VI  - 14
IP  - 2
DP  - 2007 Feb
TI  - Expression of antibodies directed to Paracoccidioides brasiliensis 
      glycosphingolipids during the course of paracoccidioidomycosis treatment.
PG  - 150-6
AB  - Paracoccidioidomycosis (PCM) is a granulomatous disease caused by the dimorphic 
      fungus Paracoccidioides brasiliensis. The immunoglobulin classes and isotypes of 
      antibodies directed to acidic glycosphingolipids (GSLs) and glucosylceramide of 
      P. brasiliensis were determined by enzyme-linked immunosorbent assay of sera from 
      31 PCM patients. The reactivities of 38 serum samples were analyzed by 
      considering the stage of treatment: before antifungal treatment (n = 10), during 
      1 to 4 months of treatment (T1-4; n = 9), during 5 to 12 months of treatment 
      (T5-12; n = 9), and posttreatment (PT; n = 10). Sera from healthy subjects (n = 
      12) were used as controls. Only the GSL Pb-1 antigen, which presents the 
      carbohydrate structure Galfbeta1-6(Manalpha1-3)Manbeta1, was reactive with the 
      PCM patient sera. The PCM patient sera did not react with Pb-2, which lacks the 
      Galf residue and which is considered the biosynthetic precursor of Pb-1, 
      indicating that the Galf residue is essential for antibody reactivity. The Pb-1 
      glycolipid from nontreated patients elicited a primary immune response with 
      immunoglobulin M (IgM) production and subsequent switching to IgG1 production. 
      The IgG1 titer increased after the start of antifungal treatment (T1-4 group), 
      and general decreases in the anti-Pb-1 antibody titers were observed after 5 
      months of treatment (T5-12 and PT groups). The Pb-1 antigen, an acidic GSL with 
      terminal Galf residue, has potential application as an elicitor of the host 
      immune response in patients with PCM.
FAU - Bertini, Silvio
AU  - Bertini S
AD  - Department of Biochemistry, Universidade Federal de Sao Paulo/Escola Paulista de 
      Medicina, Rua Botucatu, 862, Sao Paulo, SP 04023-900, Brazil.
FAU - Colombo, Arnaldo L
AU  - Colombo AL
FAU - Takahashi, Helio K
AU  - Takahashi HK
FAU - Straus, Anita H
AU  - Straus AH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061129
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Fungal)
RN  - 0 (Glycosphingolipids)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Antibodies, Fungal/*biosynthesis/blood
MH  - Glycosphingolipids/*immunology
MH  - Humans
MH  - Middle Aged
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology/therapy
PMC - PMC1797792
EDAT- 2006/12/01 09:00
MHDA- 2007/04/28 09:00
PMCR- 2007/06/01
CRDT- 2006/12/01 09:00
PHST- 2006/12/01 09:00 [pubmed]
PHST- 2007/04/28 09:00 [medline]
PHST- 2006/12/01 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - CVI.00285-06 [pii]
AID - 0285-06 [pii]
AID - 10.1128/CVI.00285-06 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2007 Feb;14(2):150-6. doi: 10.1128/CVI.00285-06. Epub 2006 
      Nov 29.