PMID- 17135673
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20240109
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 85
IP  - 12
DP  - 2006 Dec
TI  - Chinese herbal ingredients are effective immune stimulators for chickens infected 
      with the Newcastle disease virus.
PG  - 2169-75
AB  - This study was conducted to determine the efficacy of 4 Chinese herbal 
      ingredients (CHI) as immune stimulators for an active vaccine in chickens using 
      both in vitro and in vivo assays. The CHI used were Astragalus polysaccharide 
      (APS), Isatis root polysaccharide (IRPS), Propolis polysaccharide, and Epimedium 
      flavone at various concentrations. Two hundred 14-d-old male White Roman chickens 
      were randomly divided into 10 groups. Chickens in groups 1 to 9 were inoculated 
      with the New-castle disease virus (NDV) strain IV vaccine by intranasal and 
      intraocular administration. Chickens in groups 1 to 8 were also administered 
      subcutaneously on the dorsal region of the neck with 0.5 mL of the corresponding 
      CHI at 2 doses: 29 and 58 mg/kg of BW for APS and IRPS and 7.25 and 14.5 mg/kg of 
      BW for the others, once daily for 3 successive days. In group 9 (CHI-free 
      control) and group 10 (both vaccine- and CHI-free control), chickens were 
      injected with 0.5 mL of physiological saline. New-castle disease virus-specific 
      serum hemagglutination inhibition antibody (Ab) production in immunized chickens 
      was quantified using established methods. The results indicate that a majority of 
      the CHI used at appropriate concentrations were effective in enhancing in vitro 
      proliferation of chick embryo fibroblasts in response to the NDV infection. In 
      vivo administration of CHI to vaccinated chickens (7.25 to 58 mg/kg of BW, 
      depending on type) increased serum anti-NDV hemagglutination inhibition Ab titer 
      concentrations, compared with the administration the NDV alone. For all CHI, a 
      beneficial effect on the Ab production was observed on d 21 after the initiation 
      of the vaccination. On the basis of the in vivo doses used, Propolis 
      polysaccharide and Epimedium flavone were more potent than APS and IRPS in 
      promoting the humoral immune response in the young birds (P < 0.05). 
      Collectively, these findings suggest that appropriate doses of CHI can be used as 
      novel, effective immune stimulators for chickens.
FAU - Kong, X-F
AU  - Kong XF
AD  - Institute of Traditional Chinese Veterinary Medicine, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, P.R. China, 210095.
FAU - Hu, Y-L
AU  - Hu YL
FAU - Yin, Y-L
AU  - Yin YL
FAU - Wu, G-Y
AU  - Wu GY
FAU - Rui, R
AU  - Rui R
FAU - Wang, D-Y
AU  - Wang DY
FAU - Yang, C-B
AU  - Yang CB
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Hemagglutinins)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Animals
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chickens/*immunology/*virology
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Fibroblasts/cytology/drug effects
MH  - Hemagglutinins/blood
MH  - Immunologic Factors/*therapeutic use
MH  - Male
MH  - Newcastle Disease/*drug therapy/*immunology
MH  - Newcastle disease virus
EDAT- 2006/12/01 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/12/01 09:00
PHST- 2006/12/01 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/12/01 09:00 [entrez]
AID - S0032-5791(19)42488-7 [pii]
AID - 10.1093/ps/85.12.2169 [doi]
PST - ppublish
SO  - Poult Sci. 2006 Dec;85(12):2169-75. doi: 10.1093/ps/85.12.2169.