PMID- 1713576
OWN - NLM
STAT- MEDLINE
DCOM- 19910830
LR  - 20190918
IS  - 0162-3109 (Print)
IS  - 0162-3109 (Linking)
VI  - 21
IP  - 1
DP  - 1991 Jan-Feb
TI  - Role of endoplasmic reticulum, an intracellular Ca2+ store, in histamine release 
      from rat peritoneal mast cell.
PG  - 13-21
AB  - By means of the Ca-antimonate precipitation technique, it was revealed that Ca2+ 
      accumulates in the endoplasmic reticulum (ER) of rat peritoneal mast cells. The 
      ER of rat mast cells was isolated using Percoll density gradient centrifugation, 
      and its characteristics were studied. Although the uptake of 45Ca into the ER was 
      enhanced by adenosine 5'-triphosphate (ATP) at concentrations lower than 2 mM, at 
      higher concentrations ATP induced 45Ca release from the ER, suggesting that 
      bidirectional translocation of Ca2+ takes place in the ER membrane. When apyrase 
      was added to the reaction mixture to decompose all ATP molecules, the amount of 
      45Ca in the ER was decreased, indicating that ATP is necessary to retain Ca2+ in 
      the ER. Not only inositol 1,4,5-trisphosphate (IP3) but also guanosine 
      5'-triphosphate (GTP) was effective in releasing Ca2+ from the ER at 
      concentrations higher than 2 microM, while guanosine 5'-[gamma-thio]-triphosphate 
      (GTP-gamma S), a non-hydrolysable analogue of GTP, was not effective. This may 
      indicate that a hydrolysis of GTP is necessary for Ca2+ release from the ER. 
      Intracellular Ca2+ blockers such as 
      8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8) and dantrolene sodium 
      were effective in inhibiting Ca2+ release from ER induced by IP3. The Ca2+ 
      release due to IP3 was also inhibited by flunarizine, a Ca2+ channel blocker, and 
      by oxatomide, an antiallergic drug. Since these two compounds are 
      diphenylpiperazine derivatives, it is suggested that a diphenylpiperazine moiety 
      may be effective in inhibiting Ca2+ release from the ER.
FAU - Yoshii, N
AU  - Yoshii N
AD  - Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama 
      University, Japan.
FAU - Mio, M
AU  - Mio M
FAU - Akagi, M
AU  - Akagi M
FAU - Tasaka, K
AU  - Tasaka K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunopharmacology
JT  - Immunopharmacology
JID - 7902474
RN  - 0 (Calcium Channel Blockers)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Adenosine Triphosphate/pharmacology
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Endoplasmic Reticulum/drug effects/physiology
MH  - Guanosine Triphosphate/pharmacology
MH  - Histamine Release/drug effects/*physiology
MH  - In Vitro Techniques
MH  - Male
MH  - Mast Cells/drug effects/immunology/*physiology
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 0162-3109(91)90003-H [pii]
AID - 10.1016/0162-3109(91)90003-h [doi]
PST - ppublish
SO  - Immunopharmacology. 1991 Jan-Feb;21(1):13-21. doi: 10.1016/0162-3109(91)90003-h.