PMID- 17136677
OWN - NLM
STAT- MEDLINE
DCOM- 20070206
LR  - 20160526
IS  - 0743-684X (Print)
IS  - 0743-684X (Linking)
VI  - 22
IP  - 8
DP  - 2006 Nov
TI  - Proinflammatory cytokines gene expression in skin flaps with arterial and venous 
      ischemia in rats.
PG  - 641-7
AB  - Infiltration of inflammatory cells is the crucial element in ischemia-reperfusion 
      injury of the microsurgical flap. Cytokines are a large functional group of 
      polypeptide regulatory molecules that influence the activity of various cell 
      types through autocrine and paracrine mechanisms. In this study, expression of 
      selected proinflammatory cytokines was examined in skin flaps with arterial and 
      venous ischemia in the rat model. Fifty-four Sprague-Dawley rats were used in the 
      study. The ischemia of each flap was induced by clamping its vascular pedicle for 
      6 hr. The flap was then replaced and allowed to reperfuse. All flaps were 
      biopsied immediately post-event, and at 3, 6 and 18 hr after reperfusion. 
      Expression of tumor necrosis factor (TNF-alpha), interleukin (IL-1beta), and 
      monocyte chemoattractant protein-1 (MCP-1) mRNA was determined by RT-PCR in each 
      case. The same number of skin flaps without ischemia was used for baseline gene 
      expression. Results showed that the TNF-alpha expression was significantly 
      up-regulated in the flaps with arterial ischemia at 6 hr after reperfusion. In 
      the flaps with venous ischemia, MCP-1 expression was increased with its peak 
      expression at 3 hr after reperfusion. IL-1beta expression was increased threefold 
      in the flaps subjected to venous ischemia and following reperfusion in 3 hr, but 
      the peak expression in the flap with arterial ischemia was observed at 18 hr 
      after reperfusion. This study delineated the changes in expression of these 
      proinflammatory cytokines in flaps with arterial and venous ischemia reperfusion 
      injury, and showed that cytokine expression was different in the arterial and 
      venous injuries.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Division of Plastic Surgery, University of Mississippi Medical Center, Jackson, 
      Mississippi 39216, USA.
FAU - Hu, Eric C
AU  - Hu EC
FAU - Topp, Shelby
AU  - Topp S
FAU - Lei, Manping
AU  - Lei M
FAU - Chen, Weijia
AU  - Chen W
FAU - Lineaweaver, William C
AU  - Lineaweaver WC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Reconstr Microsurg
JT  - Journal of reconstructive microsurgery
JID - 8502670
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Interleukin-1beta/metabolism
MH  - Ischemia/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*metabolism
MH  - Skin/*blood supply
MH  - Surgical Flaps/*physiology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2006/12/01 09:00
MHDA- 2007/02/07 09:00
CRDT- 2006/12/01 09:00
PHST- 2006/12/01 09:00 [pubmed]
PHST- 2007/02/07 09:00 [medline]
PHST- 2006/12/01 09:00 [entrez]
AID - 10.1055/s-2006-956238 [doi]
PST - ppublish
SO  - J Reconstr Microsurg. 2006 Nov;22(8):641-7. doi: 10.1055/s-2006-956238.