PMID- 17140385
OWN - NLM
STAT- MEDLINE
DCOM- 20070430
LR  - 20190917
IS  - 0006-2979 (Print)
IS  - 0006-2979 (Linking)
VI  - 71
IP  - 11
DP  - 2006 Nov
TI  - Mechanism of action of DNA-hydrolyzing antibodies to DNA from blood of patients 
      with systemic lupus erythematosus.
PG  - 1238-46
AB  - Four fractions of IgG antibodies to native DNA (nDNA) were obtained from blood of 
      patients with systemic lupus erythematosus (SLE). These antibodies displayed a 
      thermostable DNA-hydrolyzing activity and were different in affinity for 
      DNA-cellulose and sorption on DEAE-cellulose. DNA-hydrolyzing antibodies to nDNA 
      are metal-dependent endonucleases, cause mainly single-strand breaks in DNA, and 
      are active over a wide range of pH. By atomic-force microscopy, three-dimensional 
      images of DNA complexes with DNA-hydrolyzing antibodies to nDNA were obtained 
      with nanometer resolution, and a nonprocessive action mechanism was shown for the 
      DNase activity of antibodies to nDNA.
FAU - Nevzorova, T A
AU  - Nevzorova TA
AD  - Kazan State University, 420008 Kazan, Russia. Tatyana.Nevzorova@ksu.ru
FAU - Vinter, V G
AU  - Vinter VG
FAU - Konovalova, O A
AU  - Konovalova OA
FAU - Salakhov, M Kh
AU  - Salakhov MKh
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Circular)
RN  - 0 (DNA-cellulose)
RN  - 9004-34-6 (Cellulose)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.- (Deoxyribonucleases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibodies, Antinuclear/blood/*pharmacology
MH  - Antibody Affinity
MH  - Cellulose/analogs & derivatives/metabolism
MH  - DNA/metabolism
MH  - DNA, Circular/metabolism
MH  - Deoxyribonucleases/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Hydrolysis
MH  - Lupus Erythematosus, Systemic/blood/*immunology
MH  - Male
MH  - Middle Aged
EDAT- 2006/12/05 09:00
MHDA- 2007/05/01 09:00
CRDT- 2006/12/05 09:00
PHST- 2006/12/05 09:00 [pubmed]
PHST- 2007/05/01 09:00 [medline]
PHST- 2006/12/05 09:00 [entrez]
AID - BCM71111524 [pii]
AID - 10.1134/s0006297906110095 [doi]
PST - ppublish
SO  - Biochemistry (Mosc). 2006 Nov;71(11):1238-46. doi: 10.1134/s0006297906110095.