PMID- 1714290
OWN - NLM
STAT- MEDLINE
DCOM- 19910913
LR  - 20071114
IS  - 0214-6282 (Print)
IS  - 0214-6282 (Linking)
VI  - 35
IP  - 1
DP  - 1991 Mar
TI  - Immunodetection of the transforming growth factors beta 1 and beta 2 in the 
      developing murine palate.
PG  - 17-24
AB  - The expression of some members of the TGF beta family of genes in embryonic 
      craniofacial tissue suggests a functional role for these molecules in orofacial 
      development. In other systems, TGF beta 1 and TGF beta 2 have been shown to 
      regulate cell proliferation and extracellular matrix metabolism, processes 
      critical to normal development of the secondary palate. We have thus determined 
      the amount and tissue distribution of both TGF beta 1 and TGF beta 2 in embryonic 
      palatal tissue. Cellular extracts of murine embryonic palatal tissue from days 
      12, 13 and 14 of gestation were assayed for the presence of TGF beta 1 and TGF 
      beta 2 by immunoprecipitation. Physiological levels, ranging from 0.05-20 
      ng/micrograms protein, of both growth factors were detected in all tissues 
      examined. Immunostaining with antibodies directed against either TGF beta 1 or 
      TGF beta 2 demonstrated the presence of these growth factors in palatal 
      epithelium and mesenchyme early during palatal development (gestational day [GD] 
      12) a period characterized by tissue growth. On GDs 13 and 14, characterized by 
      palate epithelial differentiation, immunostaining for both growth factors 
      predominated in epithelial tissue. Immunostaining for TGF beta 1 and TGF beta 2 
      was also intense in mesenchyme surrounding tooth germs and in perichondrium. 
      Chondrocytes and cartilage extracellular matrix did not stain for either TGF beta 
      1 or beta 2. Combined with existing evidence for the presence of functional 
      receptors for the transforming growth factor-beta s in the developing palate, 
      these results provide rationale for studies designed to clarify a specific role 
      for these signalling molecules in palate epithelial differentiation and/or 
      epithelial-mesenchymal interactions.
FAU - Gehris, A L
AU  - Gehris AL
AD  - Department of Anatomy, Jefferson Medical College, Thomas Jefferson University, 
      Philadelphia, PA 19107.
FAU - D'Angelo, M
AU  - D'Angelo M
FAU - Greene, R M
AU  - Greene RM
LA  - eng
GR  - DE08199/DE/NIDCR NIH HHS/United States
GR  - DE09540/DE/NIDCR NIH HHS/United States
GR  - HD07326/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
RN  - 0 (Protein Sorting Signals)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Epithelium/chemistry
MH  - Female
MH  - Male
MH  - Mesoderm/physiology
MH  - Mice
MH  - Palate/chemistry/*embryology
MH  - Precipitin Tests
MH  - Pregnancy
MH  - Protein Sorting Signals/genetics
MH  - RNA, Messenger/analysis
MH  - Staining and Labeling
MH  - Transforming Growth Factor beta/*analysis/genetics
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
PST - ppublish
SO  - Int J Dev Biol. 1991 Mar;35(1):17-24.