PMID- 17143527
OWN - NLM
STAT- MEDLINE
DCOM- 20070131
LR  - 20190606
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 30
IP  - 1
DP  - 2007 Jan
TI  - UV light blocks EGFR signalling in human cancer cell lines.
PG  - 181-5
AB  - UV light excites aromatic residues, causing these to disrupt nearby disulphide 
      bridges. The EGF receptor is rich in aromatic residues near the disulphide 
      bridges. Herein we show that laser-pulsed UV illumination of two different 
      skin-derived cancer cell lines i.e. Cal-39 and A431, which both overexpress the 
      EGF receptor, leads to arrest of the EGFR signaling pathway. The phosphorylation 
      status of the receptor and the level of phosphorylated downstream signaling 
      molecules i.e. AKT and the mitogen activated protein kinases (MAPKs) ERK1 and 2 
      is detected by Western blotting using phosphospecific antibodies. There was a 
      threshold level, below which the receptor could not be blocked. In addition, 
      illumination caused the cells to upregulate the cyclin-dependent kinase inhibitor 
      p21WAF1, irrespective of the p53 status. Since the EGF receptor is often 
      overexpressed in cancers and other proliferative skin disorders, it might be 
      possible to significantly reduce the proliferative potential of these cells 
      making them good targets for laser-pulsed UV light treatment.
FAU - Olsen, B B
AU  - Olsen BB
AD  - Institute for Biochemistry and Molecular Biology, University of Southern Denmark, 
      5230 Odense M, Denmark.
FAU - Neves-Petersen, M T
AU  - Neves-Petersen MT
FAU - Klitgaard, S
AU  - Klitgaard S
FAU - Issinger, O G
AU  - Issinger OG
FAU - Petersen, S B
AU  - Petersen SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Dose-Response Relationship, Radiation
MH  - ErbB Receptors/*physiology/*radiation effects
MH  - Humans
MH  - Lasers
MH  - Signal Transduction/*radiation effects
MH  - Skin Neoplasms
MH  - *Ultraviolet Rays
EDAT- 2006/12/05 09:00
MHDA- 2007/02/01 09:00
CRDT- 2006/12/05 09:00
PHST- 2006/12/05 09:00 [pubmed]
PHST- 2007/02/01 09:00 [medline]
PHST- 2006/12/05 09:00 [entrez]
AID - 10.3892/ijo.30.1.181 [doi]
PST - ppublish
SO  - Int J Oncol. 2007 Jan;30(1):181-5. doi: 10.3892/ijo.30.1.181.