PMID- 17143902
OWN - NLM
STAT- MEDLINE
DCOM- 20070302
LR  - 20131121
IS  - 1050-9631 (Print)
IS  - 1050-9631 (Linking)
VI  - 17
IP  - 1
DP  - 2007
TI  - Neonatal isolation impairs neurogenesis in the dentate gyrus of the guinea pig.
PG  - 78-91
AB  - In the current study we examined the effects of early isolation rearing on cell 
      proliferation, survival and differentiation in the dentate gyrus of the guinea 
      pig. Animals were assigned to either a standard (control) or an isolated 
      environment a few days after birth (P5-P6), taking advantage of the precocious 
      independence from maternal care of the guinea pig. On P14-P17 animals received 
      one daily bromodeoxyuridine injection, to label dividing cells, and were 
      sacrificed either on P18, to evaluate cell proliferation or on P45, to evaluate 
      cell survival and differentiation. In P18 isolated animals we found a reduced 
      cell proliferation (-35%) compared to controls and a lower expression of 
      brain-derived neurotrophic factor (BDNF). Though in absolute terms P45 isolated 
      animals had less surviving cells, they showed no differences in survival rate and 
      phenotype percent distribution compared to controls. Looking at the location of 
      the new neurons, we found that while in control animals 76% of them had migrated 
      to the granule cell layer, in isolated animals only 55% of the new neurons had 
      reached this layer. Examination of radial glia cells of P18 and P45 animals by 
      vimentin immunohistochemistry showed that in isolated animals radial glia cells 
      were reduced in density and had less and shorter processes. Granule cell count 
      revealed that P45 isolated animals had less (-42%) granule cells than controls. 
      Results show that isolation rearing reduces hippocampal cell proliferation, 
      likely by reducing BDNF expression and hampers migration of the new neurons to 
      the granule cell layer, likely by altering density/morphology of radial glia 
      cells. The large reduction in granule cell number following isolation rearing 
      emphasizes the role of environmental cues as relevant modulators of neurogenesis.
FAU - Rizzi, Simona
AU  - Rizzi S
AD  - Dipartimento di Fisiologia Umana e Generale, Universita di Bologna, Bologna, 
      Italy.
FAU - Bianchi, Patrizia
AU  - Bianchi P
FAU - Guidi, Sandra
AU  - Guidi S
FAU - Ciani, Elisabetta
AU  - Ciani E
FAU - Bartesaghi, Renata
AU  - Bartesaghi R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hippocampus
JT  - Hippocampus
JID - 9108167
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Vimentin)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Body Weight/physiology
MH  - Brain-Derived Neurotrophic Factor/metabolism/pharmacology
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Count/methods
MH  - Cell Death/physiology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Proliferation
MH  - Dentate Gyrus/*cytology/growth & development
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Guinea Pigs
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling/methods
MH  - Male
MH  - Neurons/drug effects/*physiology
MH  - Organ Size
MH  - Organogenesis/*physiology
MH  - Phosphopyruvate Hydratase/metabolism
MH  - *Social Isolation
MH  - Vimentin/metabolism
EDAT- 2006/12/05 09:00
MHDA- 2007/03/03 09:00
CRDT- 2006/12/05 09:00
PHST- 2006/12/05 09:00 [pubmed]
PHST- 2007/03/03 09:00 [medline]
PHST- 2006/12/05 09:00 [entrez]
AID - 10.1002/hipo.20247 [doi]
PST - ppublish
SO  - Hippocampus. 2007;17(1):78-91. doi: 10.1002/hipo.20247.