PMID- 17145958
OWN - NLM
STAT- MEDLINE
DCOM- 20070312
LR  - 20181113
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 203
IP  - 13
DP  - 2006 Dec 25
TI  - Dynamic imaging of dendritic cell extension into the small bowel lumen in 
      response to epithelial cell TLR engagement.
PG  - 2841-52
AB  - Cells lining the gastrointestinal tract serve as both a barrier to and a pathway 
      for infectious agent entry. Dendritic cells (DCs) present in the lamina propria 
      under the columnar villus epithelium of the small bowel extend processes across 
      this epithelium and capture bacteria, but previous studies provided limited 
      information on the nature of the stimuli, receptors, and signaling events 
      involved in promoting this phenomenon. Here, we use immunohistochemical as well 
      as dynamic explant and intravital two-photon imaging to investigate this issue. 
      Analysis of CD11c-enhanced green fluorescent protein (EGFP) or major 
      histocompatibility complex CII-EGFP mice revealed that the number of 
      trans-epithelial DC extensions, many with an unusual "balloon" shape, varies 
      along the length of the small bowel. High numbers of such extensions were found 
      in the proximal jejunum, but only a few were present in the terminal ileum. The 
      extensions in the terminal ileum markedly increased upon the introduction of 
      invasive or noninvasive Salmonella organisms, and chimeric mouse studies revealed 
      the key role of MyD88-dependent Toll-like receptor (TLR) signaling by 
      nonhematopoietic (epithelial) elements in the DC extension response. 
      Collectively, these findings support a model in which epithelial cell TLR 
      signaling upon exposure to microbial stimuli induces active DC sampling of the 
      gut lumen at sites distant from organized lymphoid tissues.
FAU - Chieppa, Marcello
AU  - Chieppa M
AD  - Lymphocyte Biology Section, Laboratory of Immunology, National Institute of 
      Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), 
      Bethesda, MD 20892, USA.
FAU - Rescigno, Maria
AU  - Rescigno M
FAU - Huang, Alex Y C
AU  - Huang AY
FAU - Germain, Ronald N
AU  - Germain RN
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061204
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (CCL20 protein, mouse)
RN  - 0 (CD11c Antigen)
RN  - 0 (CX3C Chemokine Receptor 1)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Chemokines, CC)
RN  - 0 (Cx3cr1 protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/genetics/metabolism
MH  - CX3C Chemokine Receptor 1
MH  - Cell Surface Extensions/drug effects/*physiology
MH  - Chemokine CCL20
MH  - Chemokines, CC/genetics/metabolism
MH  - Dendritic Cells/*cytology/immunology/microbiology
MH  - Epithelial Cells/drug effects/*immunology
MH  - Gene Expression
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Histocompatibility Antigens Class II/genetics/metabolism
MH  - Intestine, Small/cytology/*immunology/microbiology
MH  - *Laser Scanning Cytometry
MH  - Macrophage Inflammatory Proteins/genetics/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Mucous Membrane/cytology/immunology/microbiology
MH  - Myeloid Differentiation Factor 88/genetics
MH  - Oligodeoxyribonucleotides/pharmacology
MH  - Polysaccharides, Bacterial/pharmacology
MH  - Receptors, Chemokine/genetics/metabolism
MH  - Salmonella Infections/immunology/microbiology
MH  - Salmonella typhimurium/immunology
MH  - Signal Transduction/immunology
MH  - Toll-Like Receptors/agonists/*physiology
PMC - PMC2118178
EDAT- 2006/12/06 09:00
MHDA- 2007/03/14 09:00
PMCR- 2007/06/25
CRDT- 2006/12/06 09:00
PHST- 2006/12/06 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2006/12/06 09:00 [entrez]
PHST- 2007/06/25 00:00 [pmc-release]
AID - jem.20061884 [pii]
AID - 20061884 [pii]
AID - 10.1084/jem.20061884 [doi]
PST - ppublish
SO  - J Exp Med. 2006 Dec 25;203(13):2841-52. doi: 10.1084/jem.20061884. Epub 2006 Dec 
      4.