PMID- 17148675
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 169
IP  - 6
DP  - 2006 Dec
TI  - Exovesicles from human activated dendritic cells fuse with resting dendritic 
      cells, allowing them to present alloantigens.
PG  - 2127-36
AB  - Dendritic cells (DCs) can release microvesicles, but the latter's numbers, size, 
      and fate are unclear. Fluorescently labeled DCs were visualized by laser-scanning 
      microscopy. Using a Surpass algorithm, we were able to identify and quantify per 
      cell several hundred microvesicles released from the surface of stimulated DCs. 
      We show that most of these microvesicles are not of endocytic origin but result 
      from budding of the plasma membrane, hence their name, exovesicle. Using a double 
      vital staining, we show that exovesicles isolated from activated DCs can fuse 
      with the membrane of resting DCs, thereby allowing them to present alloantigens 
      to lymphocytes. We concluded that, within a few hours from their release, 
      exovesicles may amplify local or distant adaptive immunological response.
FAU - Obregon, Carolina
AU  - Obregon C
AD  - Department of Clinical Research, Institute of Anatomy, University of Bern, and 
      Clinic and Policlinic of Pneumology, University Hospital of Bern, Freiburgstr. 
      15, CH-3010 Berne, Switzerland.
FAU - Rothen-Rutishauser, Barbara
AU  - Rothen-Rutishauser B
FAU - Gitahi, Stephen Kiama
AU  - Gitahi SK
FAU - Gehr, Peter
AU  - Gehr P
FAU - Nicod, Laurent P
AU  - Nicod LP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Isoantigens)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - *Antigen Presentation
MH  - Cell Differentiation
MH  - Cell Membrane/physiology
MH  - Coculture Techniques
MH  - Cytoplasmic Vesicles/*immunology/physiology
MH  - Dendritic Cells/*immunology/ultrastructure
MH  - Humans
MH  - Isoantigens/*physiology
MH  - Lipopolysaccharides/pharmacology
MH  - Microscopy, Confocal/*methods
MH  - Monocytes/physiology
PMC - PMC1762484
EDAT- 2006/12/07 09:00
MHDA- 2007/01/17 09:00
PMCR- 2007/06/01
CRDT- 2006/12/07 09:00
PHST- 2006/12/07 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/12/07 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - S0002-9440(10)62672-7 [pii]
AID - 10.2353/ajpath.2006.060453 [doi]
PST - ppublish
SO  - Am J Pathol. 2006 Dec;169(6):2127-36. doi: 10.2353/ajpath.2006.060453.