PMID- 17150187
OWN - NLM
STAT- MEDLINE
DCOM- 20070212
LR  - 20061215
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 352
IP  - 3
DP  - 2007 Jan 19
TI  - Skeletal myosphere-derived progenitor cell transplantation promotes 
      neovascularization in delta-sarcoglycan knockdown cardiomyopathy.
PG  - 668-74
AB  - Bone marrow cells have been shown to contribute to neovascularization in ischemic 
      hearts, whereas their impaired maturation to restore the delta-sarcoglycan 
      (delta-SG) expression responsible for focal myocardial degeneration limits their 
      utility to treat the pathogenesis of cardiomyopathy. Here, we report the 
      isolation of multipotent progenitor cells from adult skeletal muscle, based on 
      their ability to generate floating-myospheres. Myosphere-derived progenitor cells 
      (MDPCs) are distinguishable from myogenic C2C12 cells and differentiate into 
      vascular smooth muscle cells and mesenchymal progeny. The mutation in the 
      delta-SG has been shown to develop vascular spasm to affect sarcolemma structure 
      causing cardiomyopathy. We originally generated delta-SD knockdown (KD) mice and 
      transplanted MDPCs into the hearts. MDPCs enhanced neoangiogenesis and restored 
      delta-SG expression in impaired vasculatures through trans-differentiation, 
      leading to improvement of cardiac function associated with paracrine effectors 
      secretion. We propose that MDPCs may be the promising progenitor cells in 
      skeletal muscle to treat delta-sarcoglycan complex mutant cardiomyopathy.
FAU - Nomura, Tetsuya
AU  - Nomura T
AD  - Department of Experimental Therapeutics, Translational Research Center, Kyoto 
      University Hospital, Kyoto 606-8507, Japan.
FAU - Ashihara, Eishi
AU  - Ashihara E
FAU - Tateishi, Kento
AU  - Tateishi K
FAU - Asada, Satoshi
AU  - Asada S
FAU - Ueyama, Tomomi
AU  - Ueyama T
FAU - Takahashi, Tomosaburo
AU  - Takahashi T
FAU - Matsubara, Hiroaki
AU  - Matsubara H
FAU - Oh, Hidemasa
AU  - Oh H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061127
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Sarcoglycans)
SB  - IM
MH  - Animals
MH  - Cardiomyopathies/*pathology/*surgery
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Myoblasts/*cytology/*transplantation
MH  - Neovascularization, Physiologic/*physiology
MH  - Sarcoglycans/genetics/metabolism
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/*cytology
MH  - Treatment Outcome
EDAT- 2006/12/08 09:00
MHDA- 2007/02/13 09:00
CRDT- 2006/12/08 09:00
PHST- 2006/11/10 00:00 [received]
PHST- 2006/11/14 00:00 [accepted]
PHST- 2006/12/08 09:00 [pubmed]
PHST- 2007/02/13 09:00 [medline]
PHST- 2006/12/08 09:00 [entrez]
AID - S0006-291X(06)02548-4 [pii]
AID - 10.1016/j.bbrc.2006.11.097 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Jan 19;352(3):668-74. doi: 
      10.1016/j.bbrc.2006.11.097. Epub 2006 Nov 27.