PMID- 17150915
OWN - NLM
STAT- MEDLINE
DCOM- 20070817
LR  - 20070919
IS  - 1746-8272 (Electronic)
IS  - 0261-3166 (Linking)
IP  - 50
DP  - 2006
TI  - Analysis of PPAR alpha function in human kidney cell line using siRNA.
PG  - 257-8
AB  - The peroxisome proliferator-activated receptors (PPARs) are ligand-activated 
      transcription factors that belong to the nuclear hormone receptor superfamily. 
      PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha 
      activates fatty acid catabolism, stimulates gluconeogenesis and ketone body 
      synthesis and is involved in the control of lipoprotein assembly. Although 
      PPARalpha is well characterized in the liver, its physiological function is 
      unknown in the kidney. To investigate the intimate function of PPARalpha in the 
      kidney, we analyzed the target gene expression in human metastatic renal cell 
      carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against 
      PPARalpha and real-time RT-PCR methods. We found that some selected genes 
      (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A 
      (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) 
      were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha 
      regulates fatty acid metabolism and body water homeostasis in this cell line.
FAU - Tachibana, Keisuke
AU  - Tachibana K
AD  - Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, 
      Suita, Osaka 565-0871, Japan.
FAU - Anzai, Naohiko
AU  - Anzai N
FAU - Ueda, Chihiro
AU  - Ueda C
FAU - Katayama, Tatsuya
AU  - Katayama T
FAU - Kirino, Takayoshi
AU  - Kirino T
FAU - Takahashi, Rika
AU  - Takahashi R
FAU - Yamasaki, Daisuke
AU  - Yamasaki D
FAU - Ishimoto, Kenji
AU  - Ishimoto K
FAU - Tanaka, Toshiya
AU  - Tanaka T
FAU - Hamakubo, Takao
AU  - Hamakubo T
FAU - Ueda, Yukihiko
AU  - Ueda Y
FAU - Arai, Hiroyuki
AU  - Arai H
FAU - Sakai, Juro
AU  - Sakai J
FAU - Kodama, Tatsuhiko
AU  - Kodama T
FAU - Doi, Takefumi
AU  - Doi T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Symp Ser (Oxf)
JT  - Nucleic acids symposium series (2004)
JID - 101259965
RN  - 0 (Fatty Acids)
RN  - 0 (PPAR alpha)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Body Water/metabolism
MH  - Cell Line, Tumor
MH  - Down-Regulation
MH  - Fatty Acids/metabolism
MH  - Humans
MH  - Kidney/*metabolism
MH  - PPAR alpha/antagonists & inhibitors/genetics/*physiology
MH  - Polymerase Chain Reaction
MH  - *RNA Interference
MH  - RNA, Small Interfering
EDAT- 2006/12/08 09:00
MHDA- 2007/08/19 09:00
CRDT- 2006/12/08 09:00
PHST- 2006/12/08 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2006/12/08 09:00 [entrez]
AID - 50/1/257 [pii]
AID - 10.1093/nass/nrl128 [doi]
PST - ppublish
SO  - Nucleic Acids Symp Ser (Oxf). 2006;(50):257-8. doi: 10.1093/nass/nrl128.