PMID- 17151208
OWN - NLM
STAT- MEDLINE
DCOM- 20070329
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 2
DP  - 2007 Feb
TI  - Relationship between cytomegalovirus DNA load in epithelial lining fluid and 
      plasma of lung transplant recipients and analysis of coinfection with 
      Epstein-Barr virus and human herpesvirus 6 in the lung compartment.
PG  - 324-8
AB  - Cytomegalovirus (CMV) is a significant cause of morbidity and mortality in lung 
      transplant recipients (LTRs). The aim of the present study was to elucidate the 
      relationship between the CMV DNA load in the lung compartment and that in plasma. 
      For CMV load determination, the level of CMV DNA in plasma and bronchoalveolar 
      lavage (BAL) samples was measured in a total of 97 paired BAL and plasma samples 
      obtained from 25 LTRs. The original virus concentration in the epithelial lining 
      fluid (ELF) was calculated from the BAL samples by correcting for dilution using 
      the urea dilution method. In addition, the load of Epstein-Barr virus (EBV) and 
      that of human herpesvirus 6 (HHV-6) DNA also were determined in BAL samples, 
      recalculated for their concentrations in the ELF, and compared with the CMV DNA 
      load. CMV DNA was found more frequently and at significantly higher levels in the 
      lung compartment than in plasma (P<0.001, Wilcoxon test), and the CMV load in the 
      ELF was associated with symptomatic CMV disease. EBV and HHV-6 were detected in 
      43.6% and 21.7% of the ELF samples, respectively. A statistically significant 
      association was found between the CMV and EBV DNA loads in the ELF (P<0.001; 
      Spearman's rho=0.651). Thus, in LTRs, determination of the CMV DNA load in the 
      lung compartment may be advantageous compared to monitoring only viremia. The 
      significant relationship between EBV and CMV DNA loads in the ELF of LTRs and its 
      clinical impact require further investigation.
FAU - Bauer, Claudia C
AU  - Bauer CC
AD  - Institute of Virology, Medical University of Vienna, Vienna, Austria. 
      claudia.bauer@meduniwien.ac.at
FAU - Jaksch, Peter
AU  - Jaksch P
FAU - Aberle, Stephan W
AU  - Aberle SW
FAU - Haber, Heinrich
AU  - Haber H
FAU - Lang, Gyoergy
AU  - Lang G
FAU - Klepetko, Walter
AU  - Klepetko W
FAU - Hofmann, Hanns
AU  - Hofmann H
FAU - Puchhammer-Stockl, Elisabeth
AU  - Puchhammer-Stockl E
LA  - eng
PT  - Journal Article
DEP - 20061206
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bronchoalveolar Lavage Fluid/*virology
MH  - Cytomegalovirus/genetics/*isolation & purification
MH  - Cytomegalovirus Infections/complications/virology
MH  - DNA, Viral/*analysis/blood
MH  - Epstein-Barr Virus Infections/complications/virology
MH  - Female
MH  - Herpesvirus 4, Human/*isolation & purification
MH  - Herpesvirus 6, Human/*isolation & purification
MH  - Humans
MH  - Lung/virology
MH  - Lung Transplantation/*adverse effects
MH  - Male
MH  - Middle Aged
MH  - Plasma/virology
MH  - Roseolovirus Infections/complications/virology
MH  - Viral Load
PMC - PMC1829057
EDAT- 2006/12/08 09:00
MHDA- 2007/03/30 09:00
PMCR- 2007/06/01
CRDT- 2006/12/08 09:00
PHST- 2006/12/08 09:00 [pubmed]
PHST- 2007/03/30 09:00 [medline]
PHST- 2006/12/08 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - JCM.01173-06 [pii]
AID - 1173-06 [pii]
AID - 10.1128/JCM.01173-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 Feb;45(2):324-8. doi: 10.1128/JCM.01173-06. Epub 2006 Dec 
      6.