PMID- 17154334
OWN - NLM
STAT- MEDLINE
DCOM- 20070405
LR  - 20091119
IS  - 0197-3851 (Print)
IS  - 0197-3851 (Linking)
VI  - 27
IP  - 1
DP  - 2007 Jan
TI  - FISH analysis of 15 chromosomes in human day 4 and 5 preimplantation embryos: the 
      added value of extended aneuploidy detection.
PG  - 55-63
AB  - OBJECTIVE: Screening for an increased number of chromosomes may improve the 
      detection of abnormal embryos and thus contribute to the capability of 
      preimplantation genetic screening (PGS) to detect the embryo(s) for transfer in 
      IVF with the best chance for a healthy child. Good-quality day 4 and 5 embryos 
      were analyzed after cryopreservation for the nine chromosomes mostly recommended 
      for screening (13, 14, 15, 16, 18, 21, 22, X and Y), next to six additional 
      chromosomes which are less well studied in this context (1, 2, 7, 6, 10 and 17). 
      METHOD: The copy numbers of 15 chromosomes were investigated by fluorescence in 
      situ hybridization (FISH) in three consecutive rounds. The proportion of 
      aneuploid and mosaic embryos was determined and compared in retrospect to results 
      in case only the recommended probe set had been analyzed. RESULTS: A total of 52 
      embryos from 29 infertile women were analyzed. Screening the embryos for six 
      additional chromosomes increased the proportion of abnormal embryos from 67 to 
      81% (P = 0.03), owing to an increase in mosaic embryos. CONCLUSION: All but one 
      of the meiotic aneuploidies found in this study would have been detected by the 
      probe set most frequently used in PGS clinics. However, aneuploid cell lines 
      originating from mitotic errors could be detected for almost all chromosomes, so 
      screening of six additional chromosomes mainly increased the proportion of mosaic 
      embryos. The added value of screening for six additional chromosomes in PGS for 
      clinical practice will remain undetermined as long as the fate of mosaic embryos 
      after transfer is unclear.
CI  - Copyright 2007 John Wiley & Sons, Ltd.
FAU - Baart, E B
AU  - Baart EB
AD  - Division of Reproductive Medicine, Department of Obstetrics and Gynaecology, 
      Erasmus MC, University Medical Center, Dr Molewaterplein 40, 3015 GD Rotterdam, 
      The Netherlands. e.b.baart@umcutrecht.nl
FAU - van den Berg, I
AU  - van den Berg I
FAU - Martini, E
AU  - Martini E
FAU - Eussen, H J
AU  - Eussen HJ
FAU - Fauser, B C J M
AU  - Fauser BC
FAU - Van Opstal, D
AU  - Van Opstal D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - *Aneuploidy
MH  - Chromosome Disorders/*diagnosis
MH  - Chromosomes/*genetics
MH  - Cryopreservation
MH  - Female
MH  - Genetic Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mosaicism/embryology
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
EDAT- 2006/12/13 09:00
MHDA- 2007/04/06 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/04/06 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - 10.1002/pd.1623 [doi]
PST - ppublish
SO  - Prenat Diagn. 2007 Jan;27(1):55-63. doi: 10.1002/pd.1623.