PMID- 17154481
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20121115
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 7
IP  - 12
DP  - 2006 Dec
TI  - Synthesis, characterization, and evaluation as transfection reagents of 
      ampholytic star copolymers: effect of star architecture.
PG  - 3505-12
AB  - Five star polymers based on the positively ionizable hydrophilic 
      2-(dimethylamino)ethyl methacrylate (DMAEMA) and the hydrophobic but hydrolyzable 
      tetrahydropyranyl methacrylate (THPMA) were prepared by group-transfer 
      polymerization (GTP) using ethylene glycol dimethacrylate (EGDMA) as the coupling 
      agent. In particular, four isomeric star copolymers (one heteroarm, two star 
      block, and the statistical star), all with a 3:1 DMAEMA:THPMA molar ratio, plus 
      one star homopolymer of DMAEMA, with degrees of polymerization of the arms equal 
      to 15, were synthesized. After star polymer preparation and preliminary 
      characterization, the THPMA units were hydrolyzed to negatively ionizable 
      hydrophilic methacrylic acid (MAA) untis, thus yielding star polyampholytes. All 
      the star polyampholytes as well as the commercially available transfection 
      reagent SuperFect were evaluated for their ability to transfect human cervical 
      HeLa cancer cells with the modified plasmid pRLSV40 bearing the enhanced green 
      fluorescent protein (EGFP) as the reporter gene. The transfection efficiency was 
      affected by star architecture. The DMAEMA15-star-MAA5 polyampholyte presented the 
      highest transfection efficiency of all the star polymers tested but lower than 
      that of SuperFect at its optimum conditions. All four star copolymers showed 
      decreased toxicity compared to the DMAEMA star homopolymer for the same amounts 
      of star polymer tested and also compared to the SuperFect at its optimum 
      conditions.
FAU - Georgiou, Theoni K
AU  - Georgiou TK
AD  - Department of Chemistry, University of Cyprus, P. O. Box 20537, 1678 Nicosia, 
      Cyprus.
FAU - Phylactou, Leonidas A
AU  - Phylactou LA
FAU - Patrickios, Costas S
AU  - Patrickios CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Methacrylates)
RN  - 0 (Polymers)
RN  - 0 (Solutions)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - O0V97PV2G1 (2-(dimethylamino)ethyl methacrylate)
SB  - IM
MH  - Green Fluorescent Proteins/genetics
MH  - HeLa Cells
MH  - Humans
MH  - Hydrolysis
MH  - Methacrylates/*chemistry
MH  - Polymers
MH  - Solutions
MH  - Transfection/*methods
EDAT- 2006/12/13 09:00
MHDA- 2007/02/21 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - 10.1021/bm060657y [doi]
PST - ppublish
SO  - Biomacromolecules. 2006 Dec;7(12):3505-12. doi: 10.1021/bm060657y.