PMID- 17156383
OWN - NLM
STAT- MEDLINE
DCOM- 20070223
LR  - 20220129
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 24
IP  - 11
DP  - 2006 Dec
TI  - Morphological and cellular changes within embryonic striatal grafts associated 
      with enriched environment and involuntary exercise.
PG  - 3223-33
AB  - Environmental enrichment (EE) and exercise have been implicated in influencing 
      behaviour and altering neuronal processes associated with cellular morphology in 
      both 'normal' and injured states of the CNS. Using a rodent model of Huntington's 
      disease, we investigated whether prolonged EE or involuntary exercise can induce 
      morphological and cellular changes within embryonic striatal transplants. Adult 
      rats were trained on the Staircase test--requiring fine motor control to reach 
      and collect reward pellets--prior to being lesioned unilaterally in the dorsal 
      neostriatum with quinolinic acid. The lesioned animals received E15 whole 
      ganglionic eminence cell suspension grafts followed by housing in EE or standard 
      cages. Half of the animals in standard cages received daily forced exercise on a 
      treadmill. The grafted animals showed significant functional recovery on both the 
      Staircase test and in drug-induced rotation. Neither the housing conditions nor 
      the training had an impact on the behaviour, with the exception of the treadmill 
      reducing the ipsilateral drug-induced rotation observed amongst the lesioned 
      animals. However, the animals housed in the EE had significantly increased 
      striatal brain-derived neurotrophic factor (BDNF) levels, and graft neurons in 
      these animals exhibited both greater spine densities and larger cell volumes. 
      Animals on forced exercise regime had reduced BDNF levels and grafted cells with 
      sparser spines. The study suggests that the context of the animal can affect the 
      plasticity of transplanted cells. Appropriately exploiting the underlying, and 
      yet unknown, mechanisms could lead the way to improved anatomical and potentially 
      functional integration of the graft.
FAU - Dobrossy, Mate D
AU  - Dobrossy MD
AD  - Brain Repair Group, School of Biosciences, Cardiff University, Museum Avenue Box 
      911, Cardiff CF10 3US, UK. dobrossymd@cf.ac.uk
FAU - Dunnett, Stephen B
AU  - Dunnett SB
LA  - eng
GR  - G0500794/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Shape/physiology
MH  - Cells, Cultured
MH  - Corpus Striatum/*cytology/embryology/*transplantation
MH  - Dendritic Spines/physiology/ultrastructure
MH  - Disease Models, Animal
MH  - Environment, Controlled
MH  - Female
MH  - Gait Disorders, Neurologic/therapy
MH  - Graft Survival/*physiology
MH  - Huntington Disease/*therapy
MH  - Motor Activity/physiology
MH  - Physical Conditioning, Animal/*physiology
MH  - Physical Stimulation/methods
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function/physiology
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/*cytology/physiology
MH  - Treatment Outcome
MH  - Up-Regulation/physiology
EDAT- 2006/12/13 09:00
MHDA- 2007/02/24 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/02/24 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - EJN5182 [pii]
AID - 10.1111/j.1460-9568.2006.05182.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2006 Dec;24(11):3223-33. doi: 10.1111/j.1460-9568.2006.05182.x.