PMID- 17156739
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20061212
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 38
IP  - 1
DP  - 2007 Jan-Feb
TI  - Effect of temperature and relative humidity on the stability of infectious 
      porcine reproductive and respiratory syndrome virus in aerosols.
PG  - 81-93
AB  - The objective of this experiment was to describe the stability of airborne 
      infectious porcine reproductive and respiratory syndrome virus (PRRSV) as a 
      function of temperature and relative humidity. A cloud of infectious PRRSV was 
      aerosolized using 24-jet Collison nebulizer into a dynamic aerosol toroid (DAT) 
      maintained at a specific temperature and relative humidity. The PRRSV cloud 
      within the DAT was sampled repeatedly over time using SKC BioSampler impingers 
      and the total viral RNA (RT-PCR) and concentration of infectious PRRSV (TCID50) 
      in the air samples was determined. As measured by quantitative RT-PCR, PRRSV RNA 
      was stable under the conditions evaluated in this study. Thus, a comparison of 
      viral RNA and Rhodamine B dye, a physical tracer, found no significant difference 
      in the slopes of the lines. Titers of infectious virus were plotted by time and 
      the half-life (T1/2) of infectious PRRSV was calculated using linear regression 
      analysis. An analysis of the results showed that aerosolized PRRSV was more 
      stable at lower temperatures and/or lower relative humidity, but temperature had 
      a greater effect on the T1/2 of PRRSV than relative humidity. Based on these 
      results, an equation was derived to predict the T1/2 of infectious airborne PRRSV 
      for any combination of environmental temperature and relative humidity.
FAU - Hermann, Joseph
AU  - Hermann J
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1250, USA.
FAU - Hoff, Steve
AU  - Hoff S
FAU - Munoz-Zanzi, Claudia
AU  - Munoz-Zanzi C
FAU - Yoon, Kyoung-Jin
AU  - Yoon KJ
FAU - Roof, Michael
AU  - Roof M
FAU - Burkhardt, Anna
AU  - Burkhardt A
FAU - Zimmerman, Jeffrey
AU  - Zimmerman J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061208
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
RN  - 0 (Aerosols)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *Aerosols
MH  - *Humidity
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - RNA, Viral/analysis
MH  - *Temperature
MH  - Time Factors
EDAT- 2006/12/13 09:00
MHDA- 2007/02/28 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/07/07 00:00 [received]
PHST- 2006/09/13 00:00 [accepted]
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - v07007 [pii]
AID - 10.1051/vetres:2006044 [doi]
PST - ppublish
SO  - Vet Res. 2007 Jan-Feb;38(1):81-93. doi: 10.1051/vetres:2006044. Epub 2006 Dec 8.