PMID- 17156861
OWN - NLM
STAT- MEDLINE
DCOM- 20070411
LR  - 20131121
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 140
IP  - 1-2
DP  - 2007 Mar
TI  - Real-time PCR determination of human herpesvirus 6 antiviral drug susceptibility.
PG  - 25-31
AB  - A quantitative real-time PCR assay was developed to determine the antiviral drug 
      susceptibility of human herpesvirus 6 (HHV-6). After short-term culture of the 
      virus, HHV-6 isolates' susceptibility to the antiviral ganciclovir (GCV) was 
      determined by measuring the HHV-6 variant B (HHV-6B) DNA levels in culture 
      supernatants and infected cells using real-time PCR. A total of 12 
      well-characterized GCV-sensitive or -resistant strains and clinical isolates were 
      used. This new assay with real-time PCR readout permitted the rapid (3 days), 
      objective, and reproducible determination of HHV-6 drug susceptibilities with no 
      need for stringent control of the initial multiplicity of infection. Furthermore, 
      the real-time PCR assay results showed good correlation (rs=0.95) with those from 
      the conventional TCID50 (50% tissue culture infecting dose) reduction assay 
      (TRA). Thus, the real-time PCR assay described in this report was found to be a 
      suitable quantitative method for determining the susceptibility of HHV-6 to 
      antiviral drugs. It is faster and simpler than the TRA, and it is amenable to use 
      in the routine diagnostic virology laboratory.
FAU - Isegawa, Yuji
AU  - Isegawa Y
AD  - Department of Infectious Disease Control, Graduate School of Medicine, Osaka 
      University, Suita, Osaka 565-0871, Japan. isegawa@bact.med.osaka-u.ac.jp
FAU - Takemoto, Masaya
AU  - Takemoto M
FAU - Yamanishi, Koichi
AU  - Yamanishi K
FAU - Ohshima, Atsushi
AU  - Ohshima A
FAU - Sugimoto, Nakaba
AU  - Sugimoto N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20061206
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Viral)
RN  - P9G3CKZ4P5 (Ganciclovir)
SB  - IM
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line, Tumor
MH  - DNA, Viral/analysis
MH  - Ganciclovir/*pharmacology
MH  - Herpesvirus 6, Human/classification/*drug effects/isolation & purification
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2006/12/13 09:00
MHDA- 2007/04/12 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/06/05 00:00 [received]
PHST- 2006/10/19 00:00 [revised]
PHST- 2006/10/23 00:00 [accepted]
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/04/12 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - S0166-0934(06)00377-6 [pii]
AID - 10.1016/j.jviromet.2006.10.009 [doi]
PST - ppublish
SO  - J Virol Methods. 2007 Mar;140(1-2):25-31. doi: 10.1016/j.jviromet.2006.10.009. 
      Epub 2006 Dec 6.