PMID- 17157716
OWN - NLM
STAT- MEDLINE
DCOM- 20070126
LR  - 20161019
IS  - 0741-8329 (Print)
IS  - 0741-8329 (Linking)
VI  - 40
IP  - 1
DP  - 2006 Aug
TI  - Protein expression changes in the nucleus accumbens and amygdala of inbred 
      alcohol-preferring rats given either continuous or scheduled access to ethanol.
PG  - 3-17
AB  - Chronic ethanol (EtOH) drinking produces neuronal alterations within the limbic 
      system. To investigate changes in protein expression levels associated with EtOH 
      drinking, inbred alcohol-preferring (iP) rats were given one of three EtOH access 
      conditions in their home-cages: continuous ethanol (CE: 24h/day, 7days/week 
      access to EtOH), multiple scheduled access (MSA: four 1-h sessions during the 
      dark cycle/day, 5 days/week) to EtOH, or remained EtOH-naive. Both MSA and CE 
      groups consumed between 6 and 6.5g of EtOH/kg/day after the 3rd week of access. 
      On the first day of EtOH access for the seventh week, access was terminated at 
      the end of the fourth MSA session for MSA rats and the corresponding time point 
      (2300h) for CE rats. Ten h later, the rats were decapitated, brains extracted, 
      the nucleus accumbens (NAcc) and amygdala (AMYG) microdissected, and protein 
      isolated for 2-dimensional gel electrophoretic analyses. In the NAcc, MSA altered 
      expression levels for 12 of the 14 identified proteins, compared with controls, 
      with six of these proteins altered by CE access, as well. In the AMYG, CE access 
      changed expression levels for 22 of the 27 identified proteins, compared with 
      controls, with 8 of these proteins altered by MSA, as well. The proteins could be 
      grouped into functional categories of chaperones, cytoskeleton, intracellular 
      communication, membrane transport, metabolism, energy production, or 
      neurotransmission. Overall, it appears that EtOH drinking and the conditions 
      under which EtOH is consumed, differentially affect protein expression levels 
      between the NAcc and AMYG. This may reflect differences in neuroanatomical and/or 
      functional characteristics associated with EtOH self-administration and possibly 
      withdrawal, between these two brain structures.
FAU - Bell, R L
AU  - Bell RL
AD  - Institute of Psychiatric Research and Department of Psychiatry, Indiana 
      University School of Medicine, Indianapolis, IN 46202, USA. ribell@iupui.edu
FAU - Kimpel, M W
AU  - Kimpel MW
FAU - Rodd, Z A
AU  - Rodd ZA
FAU - Strother, W N
AU  - Strother WN
FAU - Bai, F
AU  - Bai F
FAU - Peper, C L
AU  - Peper CL
FAU - Mayfield, R D
AU  - Mayfield RD
FAU - Lumeng, L
AU  - Lumeng L
FAU - Crabb, D W
AU  - Crabb DW
FAU - McBride, W J
AU  - McBride WJ
FAU - Witzmann, F A
AU  - Witzmann FA
LA  - eng
GR  - AA013521/AA/NIAAA NIH HHS/United States
GR  - AA016652/AA/NIAAA NIH HHS/United States
GR  - AA007462/AA/NIAAA NIH HHS/United States
GR  - AA007611/AA/NIAAA NIH HHS/United States
GR  - U01 AA013522/AA/NIAAA NIH HHS/United States
GR  - AA013522/AA/NIAAA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Alcohol
JT  - Alcohol (Fayetteville, N.Y.)
JID - 8502311
RN  - 0 (Biomarkers)
RN  - 0 (Central Nervous System Depressants)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Enzymes)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Molecular Chaperones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proteins)
RN  - 3K9958V90M (Ethanol)
SB  - IM
CIN - Alcohol. 2006 Aug;40(1):1-2. PMID: 17157715
MH  - Alcohol Drinking/*metabolism
MH  - Amygdala/drug effects/*metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Central Nervous System Depressants/*administration & dosage/pharmacology
MH  - Cytoskeletal Proteins/metabolism
MH  - Drug Administration Schedule
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Enzymes/metabolism
MH  - Ethanol/*administration & dosage/pharmacology
MH  - Female
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Membrane Transport Proteins/metabolism
MH  - Molecular Chaperones/metabolism
MH  - Nerve Tissue Proteins/metabolism
MH  - Nucleus Accumbens/drug effects/*metabolism
MH  - Peptide Mapping
MH  - Proteins/*metabolism
MH  - Proteomics/methods
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Self Administration
MH  - Time Factors
EDAT- 2006/12/13 09:00
MHDA- 2007/01/27 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/08/21 00:00 [received]
PHST- 2006/10/04 00:00 [revised]
PHST- 2006/10/04 00:00 [accepted]
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/01/27 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - S0741-8329(06)00207-2 [pii]
AID - 10.1016/j.alcohol.2006.10.001 [doi]
PST - ppublish
SO  - Alcohol. 2006 Aug;40(1):3-17. doi: 10.1016/j.alcohol.2006.10.001.