PMID- 17161848
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 80
IP  - 10
DP  - 2007 Feb 13
TI  - Antiestrogenic activity of vitamin A in in vivo uterotrophic assay.
PG  - 945-9
AB  - All-trans-retinoic acid (ATRA), the primary active metabolite of vitamin A, was 
      examined for its antiestrogenic activity in rats using an in vivo uterotrophic 
      assay. All rats were ovariectomized 2 weeks prior to receiving 5 mg/kg/day ATRA 
      or 0.3 micro g/kg/day ethynyl estradiol (EE) subcutaneously once a day for 3 
      consecutive days. Rats were sacrificed 1, 3, 6, 12 or 24 h after the last 
      treatment. EE increased uterine weight and the coinjection of ATRA with EE 
      significantly suppressed this effect 3 and 24 h after treatment. mRNA expression 
      was examined during this 24-h period and the mRNA expression levels of estrogen 
      receptor alpha (ER alpha), retinoic acid receptor beta (RAR beta), retinoid X 
      receptor gamma (RXR gamma) and cellular retinol-binding protein I (CRBP I) were 
      found to have significantly increased in the ATRA+EE group compared with those in 
      the EE group. This is the first report on the antiestrogenic activity of ATRA 
      determined using an in vivo adult rat uterotrophic assay. The up-regulation of 
      RAR or RXR mRNA expression level was probably responsible for the antiestrogenic 
      activity of ATRA.
FAU - Koda, Tomoko
AU  - Koda T
AD  - Division of Environmental Health Sciences, Department of Social Medicine, Faculty 
      of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, Japan. 
      tk760131@med.miyazaki-u.ac.jp
FAU - Imai, Hideki
AU  - Imai H
FAU - Morita, Masatoshi
AU  - Morita M
LA  - eng
PT  - Journal Article
DEP - 20061122
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Estradiol Congeners)
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Retinoic Acid)
RN  - 0 (Retinoid X Receptor gamma)
RN  - 0 (retinoic acid binding protein I, cellular)
RN  - 423D2T571U (Ethinyl Estradiol)
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Animals
MH  - Estradiol Congeners/pharmacology
MH  - *Estrogen Antagonists
MH  - Estrogen Receptor alpha/biosynthesis
MH  - Estrogen Receptor beta/biosynthesis
MH  - Ethinyl Estradiol/pharmacology
MH  - Female
MH  - Organ Size/drug effects
MH  - Ovariectomy
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Retinoic Acid/biosynthesis
MH  - Retinoid X Receptor gamma/biosynthesis
MH  - Tretinoin/*pharmacology
MH  - Up-Regulation/drug effects
MH  - Uterus/*drug effects
EDAT- 2006/12/13 09:00
MHDA- 2007/03/21 09:00
CRDT- 2006/12/13 09:00
PHST- 2006/06/06 00:00 [received]
PHST- 2006/11/01 00:00 [revised]
PHST- 2006/11/16 00:00 [accepted]
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
AID - S0024-3205(06)00893-9 [pii]
AID - 10.1016/j.lfs.2006.11.026 [doi]
PST - ppublish
SO  - Life Sci. 2007 Feb 13;80(10):945-9. doi: 10.1016/j.lfs.2006.11.026. Epub 2006 Nov 
      22.