PMID- 17162359
OWN - NLM
STAT- MEDLINE
DCOM- 20070215
LR  - 20081120
IS  - 1740-2522 (Print)
IS  - 1740-2530 (Electronic)
IS  - 1740-2522 (Linking)
VI  - 13
IP  - 2-4
DP  - 2006 Jun-Dec
TI  - Apoptosis and redistribution of the Ro autoantigen in Balb/c mouse like in 
      subacute cutaneous lupus erythematosus.
PG  - 163-6
AB  - In subacute cutaneous lupus eryhematosus (SCLE) the cutaneous antigens constitute 
      the main source of Ro and La autoantigens. The aim of this investigation was to 
      demonstrate if UV light increases the availability of Ro autoantigen in the skin, 
      also the blocking effect of Ac-DEVD-CMK a caspase inhibitor was assessed. For 
      this purpose newborn Balb/c mice were UVB irradiated (5-30 mJ/cm(2)) equivalent 
      to a moderate to severe sunburn. Animals were injected with monoclonal anti-Ro 
      antibodies from SCLE patients. Apoptosis was also induced by anti-Fas antibody 
      injection. Skin samples were examined by direct immunofluoresence, by TUNEL, and 
      the expression of caspase 3 by RT-PCR. Major findings of present studies were: 1. 
      UVB irradiation and anti-Fas induced apoptosis of keratinocytes. 2. Apoptosis 
      redistribute the Ro antigen on cell surface and is better triggered by Ro 
      antibody. 3. The caspase 3 inhibitor Ac-DEVD-CMK decreases the availability of Ro 
      autoantigen in epidermis and prevents deposition of anti-Ro. In conclusion, the 
      caspase pathway would be blocked to avoid anti-Ro deposition along skin; this 
      finding would be a prospect in the treatment of SCLE patients.
FAU - Herrera-Esparza, Rafael
AU  - Herrera-Esparza R
AD  - Department of Immunology, Centro de Biologia Experimental, Universidad Autonoma 
      de Zacatecas, Zacatecas, Mexico. herrerar@intranet.uaz.edu.mx
FAU - Villalobos, Ricardo
AU  - Villalobos R
FAU - Bollain-Y-Goytia, Juan-Jose
AU  - Bollain-Y-Goytia JJ
FAU - Ramirez-Sandoval, Roxana
AU  - Ramirez-Sandoval R
FAU - Sanchez-Rodriguez, Sergio H
AU  - Sanchez-Rodriguez SH
FAU - Pacheco-Tovar, Guadalupe
AU  - Pacheco-Tovar G
FAU - Avalos-Diaz, Esperanza
AU  - Avalos-Diaz E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Egypt
TA  - Clin Dev Immunol
JT  - Clinical & developmental immunology
JID - 101183692
RN  - 0 (Amino Acid Chloromethyl Ketones)
RN  - 0 (Autoantibodies)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (SS-A antigen)
RN  - 0 (benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone)
SB  - IM
MH  - Amino Acid Chloromethyl Ketones/pharmacology
MH  - Animals
MH  - *Apoptosis/physiology
MH  - Autoantibodies/*administration & dosage
MH  - Dose-Response Relationship, Radiation
MH  - Lupus Erythematosus, Cutaneous/immunology/*metabolism/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Photosensitivity Disorders/*etiology
MH  - Ribonucleoproteins/immunology/*metabolism
MH  - Skin/drug effects/immunology
MH  - *Ultraviolet Rays
PMC - PMC2270769
EDAT- 2006/12/13 09:00
MHDA- 2007/02/16 09:00
PMCR- 2006/06/01
CRDT- 2006/12/13 09:00
PHST- 2006/12/13 09:00 [pubmed]
PHST- 2007/02/16 09:00 [medline]
PHST- 2006/12/13 09:00 [entrez]
PHST- 2006/06/01 00:00 [pmc-release]
AID - N741KP4580Q5263M [pii]
AID - 10.1080/17402520600876796 [doi]
PST - ppublish
SO  - Clin Dev Immunol. 2006 Jun-Dec;13(2-4):163-6. doi: 10.1080/17402520600876796.