PMID- 17163378
OWN - NLM
STAT- MEDLINE
DCOM- 20070608
LR  - 20131121
IS  - 0935-8943 (Print)
IS  - 0935-8943 (Linking)
VI  - 86
IP  - 5
DP  - 2007 May
TI  - [In vitro neurite outgrowth induced by BDNF and GDNF in combination with 
      dexamethasone on cultured spiral ganglion cells].
PG  - 352-7
AB  - BACKGROUND: The efficacy of cochlear implant performance depends, among many 
      other factors, on the number of excitable spiral ganglion cells (SGCs) and the 
      nerve-electrode interface. In earlier animal studies it has been demonstrated 
      that neurotrophic factors are effective to improve SGC survival after 
      experimentally induced deafness. With regard to their anti-inflammatoric and 
      anti-proliferative effects, glucocorticoids (e. g. dexamethasone) are potentially 
      interesting therapeutic agents to reduce connective tissue formation around the 
      inserted electrode. The biological effects of a combined intervention of 
      neurotrophic factors with steroids on SGCs are unknown. Therefore the objective 
      of the study was to investigate possible trophic or even toxic effects of 
      brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic 
      factor (GDNF) and dexamethasone on neurite outgrowth of cultivated SGCs. METHODS: 
      By using dissociated postnatal spiral ganglion cells (p3-5) for cultivation in 
      the present study, the influence of the mentioned factors in various 
      concentrations and combinations on neurite outgrowth of SGCs was analysed. 
      RESULTS: Our results indicate significant trophic effects for BDNF (50 ng/ml) and 
      a combination of BDNF with dexamethasone (100 ng/ml) on SGC neurite outgrowth. In 
      contrast, single application of GDNF or dexamethasone in different concentrations 
      caused no significant changes on neurite outgrowth when compared to the control 
      condition. CONCLUSIONS: Neurite outgrowth induced by neurotrophic factors could 
      not be observed to be reduced when dexamethasone is given at the same time. 
      Therefore the demonstrated results provide a basis for further animal studies in 
      this field of research.
FAU - Stover, T
AU  - Stover T
AD  - Hals-Nasen-Ohrenklinik der Medizinischen Hochschule Hannover. 
      Stoever.Timo@MH-Hannover.de
FAU - Scheper, V
AU  - Scheper V
FAU - Diensthuber, M
AU  - Diensthuber M
FAU - Lenarz, T
AU  - Lenarz T
FAU - Wefstaedt, P
AU  - Wefstaedt P
LA  - ger
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
TT  - Neuritenwachstum in vitro durch BDNF und GDNF in Kombination mit Dexamethason auf 
      kultivierte Spiralganglienzellen.
DEP - 20061212
PL  - Germany
TA  - Laryngorhinootologie
JT  - Laryngo- rhino- otologie
JID - 8912371
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drug Combinations)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glucocorticoids)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/*pharmacology
MH  - Dexamethasone/administration & dosage/*pharmacology
MH  - Drug Combinations
MH  - Glial Cell Line-Derived Neurotrophic Factor/administration & dosage/*pharmacology
MH  - Glucocorticoids/administration & dosage/*pharmacology
MH  - Neurites/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Research
MH  - Spiral Ganglion/*cytology/*drug effects
MH  - Time Factors
EDAT- 2006/12/14 09:00
MHDA- 2007/06/09 09:00
CRDT- 2006/12/14 09:00
PHST- 2006/12/14 09:00 [pubmed]
PHST- 2007/06/09 09:00 [medline]
PHST- 2006/12/14 09:00 [entrez]
AID - 10.1055/s-2006-945005 [doi]
PST - ppublish
SO  - Laryngorhinootologie. 2007 May;86(5):352-7. doi: 10.1055/s-2006-945005. Epub 2006 
      Dec 12.