PMID- 17163841
OWN - NLM
STAT- MEDLINE
DCOM- 20070116
LR  - 20201209
IS  - 0309-0167 (Print)
IS  - 0309-0167 (Linking)
VI  - 49
IP  - 6
DP  - 2006 Dec
TI  - Diagnostic utility of FLI-1 monoclonal antibody and dual-colour, break-apart 
      probe fluorescence in situ (FISH) analysis in Ewing's sarcoma/primitive 
      neuroectodermal tumour (EWS/PNET). A comparative study with CD99 and FLI-1 
      polyclonal antibodies.
PG  - 569-75
AB  - AIMS: To compare the sensitivity and specificity of the recently commercially 
      available FLI-1 monoclonal (FLI-1m) antibody with the currently used antibodies 
      [CD99 and FLI-1 polyclonal (FLI-1p)] in the diagnosis of Ewing's 
      sarcoma/primitive neuroectodermal tumour (EWS/PNET) and to determine the 
      diagnostic value of the EWSR1 (22q12) dual-colour, break-apart rearrangement 
      probe fluorescence in situ hybridization (FISH) technique. MATERIALS AND METHODS: 
      Forty-three cases of well-documented EWS/PNET and 15 non-EWS/PNET cases were 
      retrieved from the archival files. Immunohistochemistry (IHC) for FLI-1p, FLI-1m 
      and FISH analysis was performed. RESULTS: The most sensitive and specific test 
      panel for the diagnosis of EWS/PNET is the combination of CD99 and FLI-1p. FISH 
      had a very high specificity (100%) but only a moderate sensitivity (50%). 
      CONCLUSION: The combination of CD99 and FLI-1p is the method of choice for the 
      diagnosis of EWS/PNET. EWRS1 (22q12) dual-colour, break-apart rearrangement probe 
      FISH should be used as a confirmatory test in addition to CD99 and FLI1-p due to 
      its high specificity.
FAU - Mhawech-Fauceglia, P
AU  - Mhawech-Fauceglia P
AD  - Department of Pathology and Laboratory Medicine, Roswell Park Cancer Institute, 
      Buffalo, NY 14263, USA. pmhawech1@yahoo.com
FAU - Herrmann, F
AU  - Herrmann F
FAU - Penetrante, R
AU  - Penetrante R
FAU - Beck, A
AU  - Beck A
FAU - Sait, S
AU  - Sait S
FAU - Block, A M
AU  - Block AM
FAU - Odunsi, K
AU  - Odunsi K
FAU - Fisher, J
AU  - Fisher J
FAU - Balos, L
AU  - Balos L
FAU - Cheney, R T
AU  - Cheney RT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - 0 (12E7 Antigen)
RN  - 0 (Actins)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Neoplasm)
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CD99 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (FLII protein, human)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Trans-Activators)
SB  - IM
MH  - 12E7 Antigen
MH  - Actins
MH  - Algorithms
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibodies, Neoplasm/immunology
MH  - Antigens, CD/*immunology
MH  - Biomarkers, Tumor/analysis/genetics
MH  - Bone Neoplasms/*diagnosis/genetics/immunology
MH  - Cell Adhesion Molecules/*immunology
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Microfilament Proteins/*genetics/*immunology
MH  - Neoplasm Proteins/immunology
MH  - Neuroectodermal Tumors, Primitive/*diagnosis/genetics/immunology
MH  - Predictive Value of Tests
MH  - ROC Curve
MH  - Receptors, Cytoplasmic and Nuclear/*genetics/*immunology
MH  - Sarcoma, Ewing/*diagnosis/genetics/immunology
MH  - Trans-Activators
EDAT- 2006/12/14 09:00
MHDA- 2007/01/17 09:00
CRDT- 2006/12/14 09:00
PHST- 2006/12/14 09:00 [pubmed]
PHST- 2007/01/17 09:00 [medline]
PHST- 2006/12/14 09:00 [entrez]
AID - HIS2535 [pii]
AID - 10.1111/j.1365-2559.2006.02535.x [doi]
PST - ppublish
SO  - Histopathology. 2006 Dec;49(6):569-75. doi: 10.1111/j.1365-2559.2006.02535.x.