PMID- 17163956
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 120
IP  - 3
DP  - 2007 Mar
TI  - Antigen-specific immunoglobulin E+ B cells are preferentially localized within 
      germinal centres.
PG  - 345-53
AB  - Allergen-specific immunoglobulin E (IgE) mediates immediate-type hypersensitivity 
      reactions and plays a central role in allergic diseases. Although antigen-driven 
      B-cell maturation and isotype switching occur within germinal centres (GCs), the 
      role of GCs in IgE production is poorly understood. In view of this, we 
      investigated the development of IgE-expressing cells within GCs in response to an 
      extensively characterized antigen, 2-phenyloxazolone (phOx). The phOx-specific 
      IgE-expressing cells localized within GCs 7 days after immunization, and peaked 
      in number on day 11. Surprisingly, very few IgE-positive cells were found in the 
      T-cell areas of the lymph node. Flow cytometric studies confirmed that IgE was 
      expressed by B cells and was not the result of trapping by follicular dendritic 
      cells. The specificity of the antibody response was confirmed by microdissection 
      and reverse transcription-polymerase chain reaction using phOx-specific IgE 
      primers. IgE-positive cells were primarily found within GCs while, in contrast, 
      many IgG1-positive cells could also be detected outside GCs in the T-cell areas. 
      Taken together, these data highlight the importance of GCs in the production of 
      antigen-specific IgE antibody.
FAU - Kelly, Kathleen A
AU  - Kelly KA
AD  - Department of Pathology and Laboratory Medicine, Geffen School of Medicine at 
      University of California Los Angeles, Los Angeles, CA 90095, USA.
FAU - Butch, Anthony W
AU  - Butch AW
LA  - eng
GR  - R01 AI026328/AI/NIAID NIH HHS/United States
GR  - R01-AI26328/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20061201
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (RNA, Messenger)
RN  - 1199-01-5 (2-phenyloxazolone)
RN  - 15646-46-5 (Oxazolone)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Epitopes, B-Lymphocyte/immunology
MH  - Female
MH  - Gene Expression Regulation/immunology
MH  - Germinal Center/*immunology
MH  - Haptens/immunology
MH  - Immunoglobulin E/*biosynthesis/blood/genetics
MH  - Immunoglobulin G/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microdissection/methods
MH  - Oxazolone/analogs & derivatives/immunology
MH  - RNA, Messenger/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
PMC - PMC2265882
EDAT- 2006/12/14 09:00
MHDA- 2007/04/25 09:00
PMCR- 2008/03/01
CRDT- 2006/12/14 09:00
PHST- 2006/12/14 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2006/12/14 09:00 [entrez]
PHST- 2008/03/01 00:00 [pmc-release]
AID - IMM2509 [pii]
AID - 10.1111/j.1365-2567.2006.02509.x [doi]
PST - ppublish
SO  - Immunology. 2007 Mar;120(3):345-53. doi: 10.1111/j.1365-2567.2006.02509.x. Epub 
      2006 Dec 1.