PMID- 17164346
OWN - NLM
STAT- MEDLINE
DCOM- 20070523
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 109
IP  - 8
DP  - 2007 Apr 15
TI  - Essential role of the TNF-TNFR2 cognate interaction in mouse dendritic 
      cell-natural killer cell crosstalk.
PG  - 3333-41
AB  - Dendritic cells (DCs) and natural killer (NK) cells are essential components of 
      the innate immune system and have a central role in initiation and regulation of 
      adaptive immune responses. During the early critical immune activities, DCs and 
      NK cells interact and reciprocally regulate each other via cell-cell contact. The 
      molecular mediators of the DC-NK-cell crosstalk are largely undefined. In the 
      present study, we show in mice that DC stimulation of NK-cell IFN-gamma secretion 
      requires DC membrane-bound but not secreted products; is increased by augmenting 
      the expression of DC transmembrane tumor necrosis factor (tmTNF) and NK-cell 
      transmembrane TNF receptor type 2 (tmTNFR2); is inhibited by blocking TNF or 
      TNFR2 but not TNFR1; is impaired by knocking out DC Tnf or NK-cell Tnfr2 but not 
      DC Tnfr1 or Tnfr2 and NK-cell Tnf or Tnfr1; and is restored in TNF-deficient DCs 
      by reconstituting tmTNF, but cannot be mimicked by soluble TNF. We also 
      demonstrate that DC TNF and NK-cell TNFR2 are required for DC-mediated NK-cell 
      proliferation and amplification of cytotoxic activity. These novel findings 
      provide the first evidence that DC-NK-cell crosstalk mediates enhancement of 
      NK-cell functions via triggering NK-cell tmTNFR2 by DC tmTNF.
FAU - Xu, Jun
AU  - Xu J
AD  - University of Pittsburgh Cancer Institute, Hillman Cancer Center, 5117 Centre 
      Avenue, Pittsburgh, PA, USA.
FAU - Chakrabarti, Ayan K
AU  - Chakrabarti AK
FAU - Tan, Jennifer L
AU  - Tan JL
FAU - Ge, Lisheng
AU  - Ge L
FAU - Gambotto, Andrea
AU  - Gambotto A
FAU - Vujanovic, Nikola L
AU  - Vujanovic NL
LA  - eng
GR  - P60 DE013059/DE/NIDCR NIH HHS/United States
GR  - R01 DE014775/DE/NIDCR NIH HHS/United States
GR  - 1P0 DE 13059/DE/NIDCR NIH HHS/United States
GR  - R01 DE 14775/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061212
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antiviral Agents)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type II)
RN  - 0 (Tnfrsf1a protein, mouse)
RN  - 0 (Tumor Necrosis Factors)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/immunology/pharmacology
MH  - Cell Communication/drug effects/*immunology
MH  - *Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Interferon-gamma/immunology/pharmacology
MH  - Killer Cells, Natural/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, Tumor Necrosis Factor, Type I/immunology
MH  - Receptors, Tumor Necrosis Factor, Type II/*immunology
MH  - Tumor Necrosis Factors/*immunology
PMC - PMC1852244
EDAT- 2006/12/14 09:00
MHDA- 2007/05/24 09:00
PMCR- 2008/04/15
CRDT- 2006/12/14 09:00
PHST- 2006/12/14 09:00 [pubmed]
PHST- 2007/05/24 09:00 [medline]
PHST- 2006/12/14 09:00 [entrez]
PHST- 2008/04/15 00:00 [pmc-release]
AID - S0006-4971(20)41708-2 [pii]
AID - 2006/026385 [pii]
AID - 10.1182/blood-2006-06-026385 [doi]
PST - ppublish
SO  - Blood. 2007 Apr 15;109(8):3333-41. doi: 10.1182/blood-2006-06-026385. Epub 2006 
      Dec 12.