PMID- 17164726
OWN - NLM
STAT- MEDLINE
DCOM- 20070308
LR  - 20061213
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 82
IP  - 11
DP  - 2006 Dec 15
TI  - Prediction of crossmatch outcome of highly sensitized patients by single and/or 
      multiple antigen bead luminex assay.
PG  - 1524-8
AB  - BACKGROUND: We used a solid-phase assay to identify human leukocyte antigen (HLA) 
      Class I and II specificities in highly reactive sera, and applied this 
      information to predict crossmatch outcome with greater than 90% accuracy. 
      METHODS: Sera from 20 highly sensitized end-stage renal disease patients reactive 
      to 70-100% of HLA Class I and II antigen panel were analyzed by single and/or 
      multiple antigen flow microparticle bead assay using Luminex platform (Luminex 
      assay). These sera contained antibodies directed against high frequency public 
      HLA class I and/or II epitopes accounting for 70-100% of serum's total 
      reactivity. More than 2,000 complement dependent cytotoxicity (CDC) and 200 flow 
      crossmatches (FLXM) were performed using sera from these patients and deceased 
      donor T and B lymphocytes. RESULTS: Luminex serum analysis was able to correctly 
      predict outcomes of 95% of T and B cell FLXM. In contrast, predictive values for 
      the CDC T and B cell crossmatches by Luminex serum analysis were only 77% and 
      75%, respectively. The use of serum analysis in donor selection would have 
      reduced the total number of required FLXM by more than 50%. The frequency of 
      negative T cell FLXM was 56% when donors were selected randomly; however, if 
      serum antibody analysis had been used to preselect the donors by excluding donors 
      that were likely to be positive, the frequency of corresponding negative 
      crossmatches would have increased up to 93%. CONCLUSION: This approach to donor 
      selection may allow wider geographic sharing of cadaver donor organs without 
      actually performing the crossmatch.
FAU - Vaidya, Smita
AU  - Vaidya S
AD  - Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, 
      USA. svaidya@utmb.edu
FAU - Partlow, David
AU  - Partlow D
FAU - Susskind, Brian
AU  - Susskind B
FAU - Noor, Maryam
AU  - Noor M
FAU - Barnes, Titus
AU  - Barnes T
FAU - Gugliuzza, Kristine
AU  - Gugliuzza K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Antibodies/blood
MH  - B-Lymphocytes/immunology
MH  - Cadaver
MH  - Donor Selection/*methods
MH  - Histocompatibility Antigens Class I/*analysis
MH  - Histocompatibility Antigens Class II/*analysis
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Kidney Failure, Chronic/blood/immunology
MH  - Microspheres
MH  - Retrospective Studies
MH  - Serum/*immunology
MH  - T-Lymphocytes/immunology
MH  - Tissue Donors
EDAT- 2006/12/14 09:00
MHDA- 2007/03/09 09:00
CRDT- 2006/12/14 09:00
PHST- 2006/12/14 09:00 [pubmed]
PHST- 2007/03/09 09:00 [medline]
PHST- 2006/12/14 09:00 [entrez]
AID - 00007890-200612150-00024 [pii]
AID - 10.1097/01.tp.0000246311.43634.0a [doi]
PST - ppublish
SO  - Transplantation. 2006 Dec 15;82(11):1524-8. doi: 
      10.1097/01.tp.0000246311.43634.0a.