PMID- 17165532
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20161124
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 5
DP  - 2006 Sep
TI  - [Determination of metabolites of nitrofuran antibiotics in royal jelly by high 
      performance liquid chromatography-tandem mass spectrometry].
PG  - 432-5
AB  - Nitrofurans are a group of widely used veterinary antibiotics that have been 
      banned in many countries. This has generated a great deal of interests and 
      demands for assay of nitrofurans in animal food products. To our knowledge, this 
      is the first time that a high performance liquid chromatography-tandem mass 
      spectrometry (HPLC-MS/MS) method has been successfully developed for 
      simultaneously analyzing the metabolites of four nitrofuran antibiotics 
      (furazolidone, furaltadone, nitrofurazone and nitrofurantoin) in royal jelly. 
      Trichloroacetic acid solution was used both to precipitate proteins and to 
      provide acidic reaction condition. Four isotope internal standards were utilized 
      to improve the quantitative precision. The limits of detection (LODs) were 0.03 
      microg/kg for the metabolite of furaltadone and 0.05 microg/kg for the other 
      three metabolites. The limits of quantitation were 0.20 microg/kg for the 
      metabolite of furaltadone and 0.25 microg/kg for the other three metabolites. The 
      linear range was 0.4-20 ng/mL for all the target analytes. The recoveries 
      calibrated by internal standard were in the range of 97.7%-104.8 with the 
      relative standard deviations (RSDs) of 2.7-9.7. It showed that this method could 
      meet the requirements of national monitoring plan in China and the Minimum 
      Required Performance Limits (MRPL) set by the European Union.
FAU - Ding, Tao
AU  - Ding T
AD  - National Bee-Product Reference Laboratory, Animal, Plant and Food Inspection 
      Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, 
      China. dingt@jsciq.gov.cn
FAU - Xu, Jinzhong
AU  - Xu J
FAU - Shen, Chongyu
AU  - Shen C
FAU - Wu, Bin
AU  - Wu B
FAU - Chen, Huilan
AU  - Chen H
FAU - Zhu, Chun
AU  - Zhu C
FAU - Zhao, Zengyun
AU  - Zhao Z
FAU - Jiang, Yuan
AU  - Jiang Y
FAU - Liu, Fei
AU  - Liu F
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Fatty Acids)
RN  - 0 (Nitrofurans)
RN  - 0 (Veterinary Drugs)
RN  - L497I37F0C (royal jelly)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - Fatty Acids/*chemistry
MH  - Nitrofurans/*analysis
MH  - Tandem Mass Spectrometry
MH  - Veterinary Drugs/*analysis
EDAT- 2006/12/15 09:00
MHDA- 2010/06/09 06:00
CRDT- 2006/12/15 09:00
PHST- 2006/12/15 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2006/12/15 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Sep;24(5):432-5.