PMID- 17165533
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 5
DP  - 2006 Sep
TI  - [Determination of lincosamide residues in honey using high performance liquid 
      chromatography-electrospray tandem mass spectrometry].
PG  - 436-9
AB  - An analytical method for the determination of lincosamide antibiotic residues in 
      honey was developed. In the procedure a solid-phase extraction for the isolation 
      of lincomycin and clindamycin from diluted honey samples was employed. The 
      residues of lincomycin and clindamycin were subsequently analyzed by 
      reversed-phase high performance liquid chromatography (RP-HPLC) coupled with 
      electrospray tandem mass spectrometry. Mass spectral acquisition was applied with 
      selective reaction monitoring of two diagnostic transition reactions. The 
      qualitative analysis was based on the retention time, the precursor ion and two 
      product ions, and the quantitation was carried out with intension of the 
      characteristic ion m/z 126. The linear ranges were from 1.0 microg/L to 200 
      microg/L for lincomycin and clindamycin and the correlation coefficients (r2) 
      were all greater than 0.996. The average recoveries for lincomycin and 
      clindamycin ranged from 80% to 110% in replicate sets of honey samples spiked 
      with the drug concentrations of 1.0, 5.0 and 20.0 microg/kg, and the relative 
      standard deviations (RSDs) were less than 8% for intra-day and 15% for inter-day 
      determinations. The method detection limit and quantitation limit were 0.1 
      micro/kg and 0.5 microg/kg, respectively. The method is simple and suitable for 
      routine analysis.
FAU - Xu, Jinzhong
AU  - Xu J
AD  - National Bee-Product Reference Laboratory, Animal, Plant and Food Inspection 
      Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, 
      China. xujz@jsciq.gov.cn
FAU - Wu, Bin
AU  - Wu B
FAU - Ding, Tao
AU  - Ding T
FAU - Shen, Chongyu
AU  - Shen C
FAU - Zhao, Zengyun
AU  - Zhao Z
FAU - Chen, Huilan
AU  - Chen H
FAU - Jiang, Yuan
AU  - Jiang Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Lincosamides)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Reverse-Phase
MH  - Drug Residues/*analysis
MH  - Honey/*analysis
MH  - Lincosamides/*analysis
MH  - Spectrometry, Mass, Electrospray Ionization
EDAT- 2006/12/15 09:00
MHDA- 2010/06/09 06:00
CRDT- 2006/12/15 09:00
PHST- 2006/12/15 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2006/12/15 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Sep;24(5):436-9.