PMID- 17165541
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 24
IP  - 5
DP  - 2006 Sep
TI  - [Simultaneous determination of four carotenoids in health products by 
      reversed-phase high performance liquid chromatography].
PG  - 475-8
AB  - A method was established for the determination of carotenoids (lutein, 
      zeaxanthin, lycopene, beta-carotene) in health products by reversed-phase high 
      performance liquid chromatography (RP-HPLC). The extraction methods of 
      carotenoids from different types of samples were also established. Separation and 
      quantification was achieved by using a Diamonsil C18 column (250 mm x 4.6 mm 
      i.d., 5 microm), and a mobile phase of acetonitrile and ethyl acetate were used 
      with the following gradient elution: 100% acetonitrile at the first 15 min, then 
      ethyl acetate increased to 100% in the next 10 min, at last 100% acetonitrile for 
      5 min with a flow rate of 1 mL/min, temperature of 25 degrees C and monitored 
      with a photodiode array detector at 450 nm. The external standard calibration 
      curves were used for quantification. There were good linear correlations for the 
      four carotenoids. The correlation coefficients were between 0.999 4 and 0. 999 7. 
      The detection limits were 0.4-0.5 mg/L. The recoveries of four carotenoids in 
      tablet samples were 95.3%-98.7% with the relative standard deviations (RSDs) of 
      0.89%-2.0%. The recoveries of four carotenoids in powder samples were 93.7%-98.8% 
      with RSDs of 0.89%-2.8%. The recoveries of four carotenoids in oil samples were 
      97.1%-99.2% with RSDs of 0.42%-1.2%. This method is simple, fast, accurate and 
      convenient. It can be used as one of the reliable means for quantitative 
      determination of carotenoid in health products.
FAU - Li, He
AU  - Li H
AD  - College of Food Science & Nutritional Engineering, China Agricultural University, 
      Beijing 100083, China. lh1981@126.com
FAU - Chen, Min
AU  - Chen M
FAU - Zhu, Lei
AU  - Zhu L
FAU - Liu, Lijuan
AU  - Liu L
FAU - Wang, Jingyu
AU  - Wang J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 36-88-4 (Carotenoids)
SB  - IM
MH  - Carotenoids/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Reverse-Phase
MH  - Dietary Supplements/*analysis
EDAT- 2006/12/15 09:00
MHDA- 2010/06/09 06:00
CRDT- 2006/12/15 09:00
PHST- 2006/12/15 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2006/12/15 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2006 Sep;24(5):475-8.